Cartilage Tissue Engineering
Methods and Protocols
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Protocol
Mesenchymal Stem Cells Derived from Human Adipose Tissue
Human adult mesenchymal stem cells are present in fat tissue, which can be obtained using surgical procedures such as liposuction. The multilineage capacity of mesenchymal stem cells makes them very valuable f...
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Protocol
Shear and Compression Bioreactor for Cartilage Synthesis
Mechanical forces, including hydrodynamic shear, hydrostatic pressure, compression, tension, and friction, can have stimulatory effects on cartilage synthesis in tissue engineering systems. Bioreactors capable...
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Protocol
Cartilage Tissue Engineering: What Have We Learned in Practice?
Many technologies that underpin tissue engineering as a research field were developed with the aim of producing functional human cartilage in vitro. Much of our practical experience with three-dimensional cult...
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Protocol
Human Fetal and Adult Chondrocytes
As the only cell type found in healthy adult cartilage, chondrocytes are the obvious and most direct starting point for cartilage tissue engineering. Human adult, juvenile, neonatal, and fetal chondrocytes hav...
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Metal Uptake and Nanoparticle Synthesis in Hairy Root Cultures
Hairy roots are a convenient experimental tool for investigating the interactions between plant cells and metal ions. Hairy roots of species capable of hyperaccumulating Cd and Ni have been applied to investig...
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Development of Linum flavum hairy root cultures for production of coniferin
Linum flavum hairy roots were initiated from leaf discs using Agrobacterium rhizogenes strains LBA9402 and TR105 though two other strains, 15834 and A4, were relatively ineffective for induction. Significant var...
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Article
Effect of bacitracin on growth and monoclonal antibody production by tobacco hairy roots and cell suspensions
Hairy roots and suspended cells of transformedNicotiana tabacum were used to produce full length murine IgG1 monoclonal antibody. The maximum amount of antibody accumulated per g dry weight in the hairy root cult...
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Influence of inoculum morphology on growth of Atropa belladonna hairy roots and production of tropane alkaloids
The specific growth rate of Atropa belladonna hairy roots measured in terms of root length increased by about 25% to 0.49 d-1 when the inoculum consisted of a root with the primary root tip excised. The biomass d...
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Growth kinetics and stoichiometry of Mentha citrata shooty teratomas transformed by Agrobacterium tumefaciens
Culture characteristics of genetically transformed Mentha citrata shooty teratomas were studied in liquid Murashige and Skoog and Gamborg's B5 media. Based on calculated kinetic and yield parameters, a stoichiome...
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Production of steroidal alkaloids by hairy roots of Solanum aviculare and the effect of gibberellic acid
Cultures of Solanum aviculare hairy roots were established after transformation with Agrobacterium rhizogenes A4. High levels of steroidal alkaloids measured as solasodine equivalents were produced in shake-flask...
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Production of steroidal alkaloids by hairy roots of Solanum aviculare and the effect of gibberellic acid
Cultures of Solanum aviculare hairy roots were established after transformation with Agrobacterium rhizogenes A4. High levels of steroidal alkaloids measured as solasodine equivalents were produced in shake-flask...
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Article
Effect of cell-cycle inhibition on synthesis of steroidal alkaloids by Solanum aviculare plant cells
Addition of 40 mg l-1 hydroxyurea to Solanium aviculare cultures resulted in complete inhibition of growth and an increase in the levels of steroidal alkaloids in the biomass. These results demonstrate that solas...
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Design of reactors for plant cells and organs
To be economically feasible, production of most secondary metabolites by plant-cell culture requires high cell densities. In standard reactor configurations such as stirred-tank and airlift vessels, large-scal...
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Limitations associated with conductivity measurement for monitoring growth in plant tissue culture
The suitability of conductivity measurement for monitoring growth in plant cell culture has been tested using suspended cells and genetically-transformed hairy roots of Atropa belladonna, and aggregated cells of
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Enhanced codeine and morphine production in suspended Papaver somniferum cultures after removal of exogenous hormones
Morphine and codeine accumulation in Papaver somniferum suspension cultures increased markedly after removal of hormones from the medium. Cultures developed hormone self-sufficiency without organogenesis or devel...
