Monographs on Theoretical and Applied Genetics
Volume 1 / 1975 to Volume 23 / 1998
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Conservation of RNA editing between rice and maize plastids: are most editing events dispensable?
The extent of conservation of RNA editing sites in the plastid genome of rice was determined by comparing the genomic sequence with that of the cDNA. The presence of a T in the cDNA predicted to be a C by the...
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Novel in Vitro Transcription Assay Indicates that the ACCD Nep Promoter is Contained in a 19 BP Fragment
In order to characterize promoters for the nuclear-encoded plastid RNA polymerase (NEP), we developed a novel in vitro transcription system. The assay is based on extracts from plants lacking the plastid-encoded ...
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A Transgenic Approach to Characterize the Plastid Transcription Machinery in Higher Plants
The plastid genes of higher plants are transcribed by two RNA polymerases: the plastid-encoded plastid RNA polymerase or PEP and the nuclear-encoded plastid RNA polymerase or NEP. Below is a brief summary of w...
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Plastid promoter utilization in a rice embryogenic cell culture
Plastid promoter utilization was characterized in rice by map** transcript 5′-ends in samples derived from leaves and cultured embryogenic cells. We have found that rbcL, atpB and the rRNA operon are transcrib...
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Map** of promoters for the nucleus-encoded plastid RNA polymerase (NEP) in the iojap maize mutant
Plastid genes of higher plants may be transcribed by the plastid-encoded or the nucleus-encoded plastid RNA polymerases (PEP or NEP). The objective of this study was to identify NEP promoters in maize. To sep...
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Targeted deletion of sprA from the tobacco plastid genome indicates that the encoded small RNA is not essential for pre-16S rRNA maturation in plastids
The small plastid RNA (spRNA) which includes a segment that is complementary to the pre-16S rRNA has been suggested to facilitate maturation of pre-16S rRNA in tobacco. To investigate the function of spRNA, t...
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Marker rescue from the Nicotiana tabacum plastid genome using a plastid/Escherichia coli shuttle vector
We recently reported an 868-bp plastid DNA minicircle, NICE1, that formed during transformation in a transplastomic Nicotiana tabacum line. Shuttle plasmids containing NICEI sequences were maintained extrachromos...
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Extensive homologous chloroplast DNA recombination in the pt14 Nicotiana somatic hybrid
In a previous study, six recombination sites have been confirmed in the chloroplast DNA (cpDNA) of pt14, a somatic hybrid of Nicotiana tabacum and Nicotiana plumbaginifolia. In the present study, physical map**...
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