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Article
Open AccessDevelopment of a reverse transcription recombinase polymerase amplification combined with lateral flow assay for equipment-free on-site field detection of tomato chlorotic spot virus
Tomato chlorotic spot virus (TCSV) is an economically important, thrips-transmitted, emerging member of the Orthotospovirus genus that causes significant yield loss mainly in tomatoes, but also in other vegetable...
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Article
Sequence analysis of the medium and small RNAs of impatiens necrotic spot virus reveals segment reassortment but not recombination
The complete sequence of the medium (M) and small (S) RNA genome segments were determined for twelve isolates of impatiens necrotic spot virus from eight plant species. The M- and S-RNAs of these isolates shar...
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Article
Post-transcriptional gene silencing and virus resistance in Nicotiana benthamiana expressing a Grapevine virus A minireplicon
Grapevine virus A (GVA) is closely associated with the economically important rugose-wood disease of grapevine. In an attempt to develop GVA resistance, we made a GFP-tagged GVA-minirepli...
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Article
Construction of a multiprobe for the simultaneous detection of viroids infecting citrus trees
Infections with different viroid species are common among cultivated fruit trees and grapevines, and many old-clone citrus varieties contain up to five citrus viroids (CVds) within a single tree. This paper de...
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Article
Transgenes consisting of a dsRNA of an RNAi suppressor plus the 3′ UTR provide resistance to Citrus tristeza virus sequences in Nicotiana benthamiana but not in citrus
In an attempt to utilize post-transcriptional gene silencing (PTGS) as a means to impart resistance against Citrus tristeza virus (CTV) into citrus plants, the p23 + 3′UTR sequence (p23U) of the VT strain of CTV ...
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Article
Goat horns: Platforms for viroid transmission to fruit trees?
Mechanical inoculations with contaminating tools and propagation of infected budwood were considered the main causes for the omnipresence of multiple viroid species among citrus and other Middle Eastern and Me...
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Article
A simple and efficient cryopreservation of in vitro-grown shoot tips of `Troyer' citrange [Poncirus trifoliata (L.) Raf. × Citrus sinensis (L.) Osbeck.] by encapsulation-vitrification
A simple and efficient method was developed for cryopreservation ofin vitro-grown shoot tips of `Troyer' citrange byencapsulation-vitrification. Excised shoot tips were precultured withincreasing sucrose concentr...