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Article
Relocation of dehydroquinate dehydratase to the periplasmic space improves dehydroshikimate production with Gluconobacter oxydans strain NBRC3244
3-Dehydroshikimate (3-DHS) is a key intermediate for the synthesis of various compounds, including the antiviral drug oseltamivir. The Gluconobacter oxydans strain NBRC3244 intrinsically oxidizes quinate to produ...
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Article
Three ATP-dependent phosphorylating enzymes in the first committed step of dihydroxyacetone metabolism in Gluconobacter thailandicus NBRC3255
Dihydroxyacetone (DHA), a chemical suntan agent, is produced by the regiospecific oxidation of glycerol with Gluconobacter thailandicus NBRC3255. However, this microorganism consumes DHA produced in the culture m...
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Article
Aldopentoses as new substrates for the membrane-bound, pyrroloquinoline quinone-dependent glycerol (polyol) dehydrogenase of Gluconobacter sp.
Membrane-bound, pyrroloquinoline quinone (PQQ)-dependent glycerol dehydrogenase (GLDH, or polyol dehydrogenase) of Gluconobacter sp. oxidizes various secondary alcohols to produce the corresponding ketones, such ...
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Chapter
Membrane-Bound Dehydrogenases of Acetic Acid Bacteria
One of the major key features of acetic acid bacteria is their strong oxidation ability of alcohols and sugars, resulting in quantitative production of oxidized compounds. Respiratory chains consisting of ubiq...
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Article
Reduction of l-phenylalanine in protein hydrolysates using l-phenylalanine ammonia-lyase from Rhodosporidium toruloides
l-Phenylalanine ammonia-lyase (PAL, EC 4.3.1.25) from Rhodosporidium toruloides was utilized to remove l-phenylalanine (l-Phe) from different commercial protein hydrolysates. A casein aci...
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Article
Overexpression of a type II 3-dehydroquinate dehydratase enhances the biotransformation of quinate to 3-dehydroshikimate in Gluconobacter oxydans
Shikimate and 3-dehydroshikimate are useful chemical intermediates for the synthesis of various compounds, including the antiviral drug oseltamivir. Here, we show an almost stoichiometric biotransformation of ...
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Article
High-temperature sorbose fermentation with thermotolerant Gluconobacter frateurii CHM43 and its mutant strain adapted to higher temperature
We succeeded in obtaining a strain adapted to higher temperature from a thermotolerant strain, Gluconobacter frateurii CHM43, for sorbose fermentation. The adapted strain showed higher growth and l-sorbose produc...
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Article
Characterization of thermotolerant Acetobacter pasteurianus strains and their quinoprotein alcohol dehydrogenases
We isolated several thermotolerant Acetobacter species of which MSU10 strain, identified as Acetobacter pasteurianus, could grow well on agar plates at 41°C, tolerate to 1.5% acetic acid or 4% ethanol at 39°C, si...
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Article
Coffee pulp koji of Aspergillus sojae as stable immobilized catalyst of chlorogenate hydrolase
Chlorogenate hydrolase (EC 3.1.1.42, CHase) was highly induced in mycelia of Aspergillus sojae AKU 3312 grown in Czapek medium containing either instant coffee powder or coffee pulp as inducer. No CHase formation...
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Article
Effect of NADH dehydrogenase-disruption and over-expression on respiration-related metabolism in Corynebacterium glutamicum KY9714
The function of type II NADH dehydrogenase (NDH-2) in Gram-positive Corynebacterium glutamicum was investigated by preparing strains with ndh, the NDH-2 gene, disrupted and over-expressed. Although disruption sho...
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Chapter
Chapter 4: Respiratory Chains in Acetic Acid Bacteria: Membranebound Periplasmic Sugar and Alcohol Respirations
Acetic acid bacteria are obligate aerobes, and well known to have a strong ability to oxidize ethanol, sugars or sugar alcohols to produce the corresponding sugar acids. These oxidation reactions of sugars and...
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Article
Cellular localization and metabolic function of n-butylamine-induced amine oxidases in the fungus Aspergillus niger AKU 3302
Using transmission electron microscopy, the amine oxidase activity in Aspergillus niger AKU 3302 was localized to the outer side of the cell wall but not inside the cell using the cerium perhydroxide deposition ...
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Chapter and Conference Paper
Structural Studies of a soluble monomeric Quinohemoprotein Alcohol Dehydrogenase from Pseudomonas putida HK5
We have traced the polypeptide chain at 3 Å resolution of the soluble, monomeric alcohol dehydrogenase (ADH-IIB) isolated from Pseudomonas putida. The enzyme contains a large N-terminal 8-stranded a-propeller dom...
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Chapter and Conference Paper
Electron Transport Systems for Quinohemoprotein Type II Alcohol Dehydrogenase of Pseudomonas Putida HK5
To understand the electron transport system with quinohemoprotein type II alcohol dehydrogenase (ADH; ADH IIB) inPseudomonas putidaHK5 grown on butanol, an ADHdependent electron transfer reaction was examined in ...
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Article
Quinoprotein D-glucose dehydrogenases inAcinetobacter calcoaceticus LMD 79. 41: Purification and characterization of the membrane-bound enzyme distinct from the soluble enzyme
Acinetobacter calcoaceticus is known to contain soluble and membrane-bound quinoprotein D-glucose dehydrogenases while other oxidative bacteria such asPseudomonas orGluconobacter contain only membrane-bound enzy...
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Chapter
Quinoprotein D-glucose dehydrogenases in Acinetobacter calcoaceticus LMD 79.41: Purification and characterization of the membrane-bound enzyme distinct from the soluble enzyme
Acinetobacter calcoaceticus is known to contain soluble and membrane-bound quinoprotein D-glucose dehydrogenases while other oxidative bacteria such as Pseudomonas or Gluconobacter contain only mem...
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Chapter
Binding of Pyrroloquinoline Quinone to Serum Albumin
When PQQ was mixed with serum albumin at neutral pH, a spontaneous binding of PQQ to the serum protein was observed. The resulting PQQ-albumin was fairly stable for chromatographic treatments and exhibited an ...