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Article
Cystic fibrosis transmembrane conductance regulator modulates enteric cholinergic activities and is abnormally expressed in the enteric ganglia of patients with slow transit constipation
Cystic fibrosis transmembrane conductance regulator (CFTR) was recently found in the enteric nervous system, where its role is unclear. We aimed to identify which enteric neuronal structures express CFTR, whet...
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Article
The human immune system’s response to carcinogenic and other infectious agents transmitted by mosquito vectors
It has been hypothesised that mosquitoes [Diptera: Culicidae] may play more of a role in certain cancers than is currently appreciated. Research links 33 infectious agents to cancer, 27 of which have a presenc...
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Article
Open AccessPolarization: A Key Difference between Man-made and Natural Electromagnetic Fields, in regard to Biological Activity
In the present study we analyze the role of polarization in the biological activity of Electromagnetic Fields (EMFs)/Electromagnetic Radiation (EMR). All types of man-made EMFs/EMR - in contrast to natural EMF...
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Article
PGP 9.5 distribution patterns in biopsies from early lesions of atopic dermatitis
Peripheral nerve fibres are often increased in lesional skin of atopic dermatitis (AD) patients. We attempted to study nerve fibre profiles, using PGP 9.5 as neuronal marker, in early AD lesions in 10 patients...
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Article
Combined Exposure of Peripubertal Male Rats to the Endocrine-Disrupting Compound Atrazine and Power–Frequency Electromagnetic Fields Causes Degranulation of Cutaneous Mast Cells: A New Toxic Environmental Hazard?
The effects of single and combined treatments of the endocrine-disrupting compound atrazine and the power–frequency electromagnetic fields (EMFs) were investigated on cutaneous mast cells in juvenile/peripuber...
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Article
Cutaneous PGP 9.5 distribution patterns in hidradenitis suppurativa
The aim of this study was to investigate the presence and density of the nerve fibre-marker protein gene product 9.5 (PGP 9.5), with immunohistochemistry, in skin from patients with hidradenitis suppurativa (H...
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Article
Increased nerve growth factor and its receptors in atopic dermatitis: an immunohistochemical study
Evidence suggests that neurotrophins may regulate certain immune functions and inflammation. In the present study, the localization and distribution of nerve growth factor (NGF) and its receptors were explored...
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Article
Oestrogen receptor β is present in both muscle fibres and endothelial cells within human skeletal muscle tissue
Oestrogen receptor β (ERβ) is expressed in human skeletal muscle tissue. In the present study, we have developed an immunohistochemical method to reveal if ERβ is located within the muscle fibres as well as wi...
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Article
Increased nerve growth factor- and tyrosine kinase A-like immunoreactivities in prurigo nodularis skin – an exploration of the cause of neurohyperplasia
Neurotrophins and their receptors play an important role in cutaneous nerve development and reconstruction after injury. Recent developments indicate that this group of molecules not only exert a neurotrophic...
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Article
Increased occurrence of PGP 9.5-immunoreactive epidermal Langerhans cells in rat plantar skin after sciatic nerve injury
This study examines the occurrence and distribution of epidermal dendritic cells (DCs) in cryostate sections from plantar skin in normal rats and in rats with a crush injury or neurotomy and suture of the sci...
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Article
Neuronal structural proteins, transmitters, transmitter enzymes and neuropeptides in human Meissner’s corpuscles: a reappraisal using immunohistochemistry
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Article
Serotonin in human allergic contact dermatitis. An immunohistochemical and high-performance liquid chromatographic study
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Article
A simple immunofluorescence technique for simultaneous visualization of mast cells and nerve fibers reveals selectivity and hair cycle – dependent changes in mast cell – nerve fiber contacts in murine skin
Close contacts between mast cells (MC) and nerve fibers have previously been demonstrated in normal and inflamed skin by light and electron microscopy. A key step for any study in MC-nerve intera...
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Article
The occurrence of cutaneous nerve endings and neuropeptides in vitiligo vulgaris: a case-control study
Pioneering studies both in humans and animals have demonstrated an association between the peripheral nervous system and epidermal melanocyte destruction. The presence of certain neuropeptides and neuronal str...
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Article
Hair cycle-dependent changes in mast cell histochemistry in murine skin
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Article
Histamine immunohistochemistry is superior to the conventional heparin-based routine staining methodology for investigations of human skin mast cells
Conventional studies of mast cells are limited by methodological restrictions such as a selective fixative-dependent routine staining blockage. This is thought to depend on the biochemical differences of the m...
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Article
Glutamate- and aspartate-like immunoreactivities in human normal and inflamed skin
The presence of glutamate/aspartate-like immunoreactivity was studied in normal human skin and in skin with gold-induced inflammation. In normal skin all epithelial cells were glutamate and, apparently more we...
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Article
Methodological aspects on immunohistochemistry in dermatology with special reference to neuronal markers
In an attempt to optimize the immunohistochemical procedure for visualizing neuronal markers, such as neuropeptides, in the human skin, different alternatives in all steps of the process were compared. We have...
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Article
Collateral reinnervation and expansive regenerative reinnervation by sensory axons into “foreign” denervated skin: an immunohistochemical study in the rat
Immunohistochemistry has been used to study, the capacity of different types of sensory axons in the saphenous nerve to extend into denervated glabrous skin territory after a chronic sciatic nerve lesion. In t...
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Article
Histamine immunohistochemistry: a new and highly sensitive method for studying cutaneous mast cells
Mast cells have characteristic granulae containing various glucoseaminoglycans, proteases and amines (predominantly histamine). The conventional histological methods for studying mast cells are based upon acid...
