-
Article
Structural and molecular basis of choline uptake into the brain by FLVCR2
Choline is an essential nutrient that the human body needs in vast quantities for cell membrane synthesis, epigenetic modification and neurotransmission. The brain has a particularly high demand for choline, b...
-
Article
Promiscuous G-protein activation by the calcium-sensing receptor
The human calcium-sensing receptor (CaSR) detects fluctuations in the extracellular Ca2+ concentration and maintains Ca2+ homeostasis1,2. It also mediates diverse cellular processes not associated with Ca2+ balan...
-
Article
Open AccessStructural basis of peptidoglycan synthesis by E. coli RodA-PBP2 complex
Peptidoglycan (PG) is an essential structural component of the bacterial cell wall that is synthetized during cell division and elongation. PG forms an extracellular polymer crucial for cellular viability, the...
-
Article
Structural basis of lipopolysaccharide maturation by the O-antigen ligase
The outer membrane of Gram-negative bacteria has an external leaflet that is largely composed of lipopolysaccharide, which provides a selective permeation barrier, particularly against antimicrobials1. The final ...
-
Article
Open AccessIon currents through Kir potassium channels are gated by anionic lipids
Ion currents through potassium channels are gated. Constriction of the ion conduction pathway at the inner helix bundle, the textbook gate of Kir potassium channels, has been shown to be an ineffective permeat...
-
Article
Open AccessRyR1-related myopathy mutations in ATP and calcium binding sites impair channel regulation
The type 1 ryanodine receptor (RyR1) is an intracellular calcium (Ca2+) release channel on the sarcoplasmic/endoplasmic reticulum that is required for skeletal muscle contraction. RyR1 channel activity is modulat...
-
Article
Structural basis of omega-3 fatty acid transport across the blood–brain barrier
Docosahexaenoic acid is an omega-3 fatty acid that is essential for neurological development and function, and it is supplied to the brain and eyes predominantly from dietary sources1–6. This nutrient is transpor...
-
Article
Structure of human GABAB receptor in an inactive state
The human GABAB receptor—a member of the class C family of G-protein-coupled receptors (GPCRs)—mediates inhibitory neurotransmission and has been implicated in epilepsy, pain and addiction1. A unique GPCR that is...
-
Article
Open AccessCryo-EM structure of arabinosyltransferase EmbB from Mycobacterium smegmatis
Arabinosyltransferase B (EmbB) belongs to a family of membrane-bound glycosyltransferases that build the lipidated polysaccharides of the mycobacterial cell envelope, and are targets of anti-tuberculosis drug ...
-
Article
Author Correction: Structure of human GABAB receptor in an inactive state
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
-
Article
Open AccessA constricted opening in Kir channels does not impede potassium conduction
The canonical mechanistic model explaining potassium channel gating is of a conformational change that alternately dilates and constricts a collar-like intracellular entrance to the pore. It is based on the pr...
-
Article
Intracellular calcium leak as a therapeutic target for RYR1-related myopathies
RYR1 encodes the type 1 ryanodine receptor, an intracellular calcium release channel (RyR1) on the skeletal muscle sarcoplasmic reticulum (SR). Pathogenic RYR1 variations can destabilize RyR1 leading to calcium l...
-
Article
Structural and functional characterization of the bestrophin-2 anion channel
The bestrophin family of calcium (Ca2+)-activated chloride (Cl−) channels, which mediate the influx and efflux of monovalent anions in response to the levels of intracellular Ca2+, comprises four members in mamma...
-
Article
Structure of an endosomal signaling GPCR–G protein–β-arrestin megacomplex
Classically, G-protein-coupled receptors (GPCRs) are thought to activate G protein from the plasma membrane and are subsequently desensitized by β-arrestin (β-arr). However, some GPCRs continue to signal throu...
-
Article
Open AccessStructure of the polyisoprenyl-phosphate glycosyltransferase GtrB and insights into the mechanism of catalysis
The attachment of a sugar to a hydrophobic polyisoprenyl carrier is the first step for all extracellular glycosylation processes. The enzymes that perform these reactions, polyisoprenyl-glycosyltransferases (P...
-
Article
Open AccessStructural basis for phosphatidylinositol-phosphate biosynthesis
Phosphatidylinositol is critical for intracellular signalling and anchoring of carbohydrates and proteins to outer cellular membranes. The defining step in phosphatidylinositol biosynthesis is catalysed by CDP...
-
Article
Structure of a mammalian ryanodine receptor
Ryanodine receptors (RyRs) mediate the rapid release of calcium (Ca2+) from intracellular stores into the cytosol, which is essential for numerous cellular functions including excitation–contraction coupling in m...
-
Article
Structural basis for catalysis in a CDP-alcohol phosphotransferase
The CDP-alcohol phosphotransferase (CDP-AP) family of integral membrane enzymes catalyses the transfer of a substituted phosphate group from a CDP-linked donor to an alcohol acceptor. This is an essential reac...
-
Chapter
Oligomerization at the Membrane
Cell membranes present a naturally impervious barrier to aqueous solutes, such that the physiochemical environment on either side of the lipid bilayer can substantially differ. Integral membrane proteins are e...
