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Article
Loss or Mislocalization of Aquaporin-4 Affects Diffusion Properties and Intermediary Metabolism in Gray Matter of Mice
The first aim of this study was to determine how complete or perivascular loss of aquaporin-4 (AQP4) water channels affects membrane permeability for water in the mouse brain grey matter in the steady state. T...
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Article
Changes in glutathione in the hippocampus of rats injected with kainate: depletion in neurons and upregulation in glia
The aim of the present study was to elucidate the distribution of glutathione immunoreactivity in the normal hippocampus and after kainate-induced neuronal injury. A specific antibody was used that recognizes...
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Article
Glycine, glycine receptor subunit and glycine transporters in the rat parabrachial and Kölliker-Fuse nuclei
In the present study, we investigated the expression and distribution of key molecules in the parabrachial (PB) and Kölliker-Fuse nuclei (KF) that determine glycinergic signal transduction. By means of immunoc...
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Article
Ultrastructure and immunocytochemical distribution of GABA in layer III of the rat medial entorhinal cortex following aminooxyacetic acid-induced seizures
Layer III of the entorhinal cortex (EC) is lesioned in patients with temporal lobe epilepsy (TLE). A similar neuropathology is also present in different animal models of TLE. For example, injection of the ”in...
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Article
Subcellular compartmentation of glutathione and glutathione precursors.
Selective antibodies were used to assess the cellular and subcellular localization of glutathione, and the glutathione precursors γ-glutamylcysteine, glutamate, and cysteine, in neuronal (photoreceptors) and ...
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Article
Differential distribution of the glutamate transporters GLT1 and rEAAC1 in rat cerebral cortex and thalamus: an in situ hybridization analysis
The distributions in rat cerebral cortex and thalamus of the mRNAs encoding the glutamate transporters GLT1 and rEAAC1 (a rat homologue of rabbit EAAC1) were investigated by nonautoradiographic in situ hybrid...
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Chapter
Taurine and Water Channels are Co-Localized in Renal Tubule Cells and Other Tissues
One of the best established roles of taurine is that of an osmoregulator7. Many cell types have been shown to sustain a loss of taurine upon exposure to hypoosmotic stress11 while an increased uptake of this amin...
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Article
Reduced postischemic expression of a glial glutamate transporter, GLT1, in the rat hippocampus
Perturbations of the synaptic handling of glutamate have been implicated in the pathogenesis of brain damage after transient ischemia. Notably, the ischemic episode is associated with an increased extracellula...
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Article
Immunocytochemical localization of amino acid neurotransmitter candidates in the ventral horn of the cat spinal cord: a light microscopic study
The distribution of immunoreactivities to six amino acids, possibly related to synaptic function, was investigated in the motor nucleus of the cat L7 spinal cord (laminae VII and IX) using a postembedding pero...
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Article
Effect of ischaemia and reperfusion on the extra- and intracellular distribution of glutamate, glutamine, aspartate and GABA in the rat hippocampus, with a note on the effect of the sodium channel blocker BW1003C87
The redistribution of neurotransmitter amino acids resulting from 20 min of ischaemia was studied in the rat hippocampus by quantitative, electron microscopic immunocytochemistry and by in vivo microdialysis. ...
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Article
Demonstration of glutamate-like immunoreactivity at rat neuromuscular junctions by quantitative electron microscopic immunocytochemistry
Motor nerve terminals and adjacent structures in the extensor digitorum longus and soleus muscles of young adult rats were examined for their content of glutamate by means of quantitative, electron microscopic...
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Article
An atlas of glycine- and GABA-like immunoreactivity and colocalization in the cochlear nuclear complex of the guinea pig
The distribution and colocalization of γ-aminobutyric acid (GABA)- and glycine-like immunoreactivity in the cochlear nuclear complex of the guinea pig have been studied to produce a light microscopic atlas. The m...
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Article
Immunocytochemical and circadian biochemical analysis of neuroactive amino acids in the pineal gland of the rat: effect of superior cervical ganglionectomy
Semiquantitative immunocytochemistry by immuno-gold techniques revealed differences in the spatial distribution of glutamate, glutamine, and taurine within the pineal gland, with greatest labeling over pinealo...
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Article
Differential cellular distribution of two sulphur-containing amino acids in rat cerebellum
An antiserum to homocysteic acid was raised in rabbits. Immunogens were prepared by coupling this amino acid to bovine serum albumin by means of glutaraldehyde and paraformaldehyde. When applied to semithin or...
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Article
GABA and glutamate-like immunoreactivity in processes presynaptic to afferents from hair plates on the proximal joints of the locust leg
Hair plate afferents from coxal group 1 on the meso- and prothoracic legs of the locust were backfilled with cobalt salts or HRP for light and electron microscopy. The distribution of the terminal branches of ...
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Article
GABA-like and glycine-like immunoreactivities of the cochlear root nucleus in rat
The cochlear root nucleus is part of the cochlear nuclear complex in small rodents. Its cells, the large root neurons, have a superficial resemblance to the globular neurons of the ventral cochlear nucleus. It...
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Article
GABA, glycine, aspartate, glutamate and taurine in the vestibular nuclei: an immunocytochemical investigation in the cat
The distributions of five amino acids with well-established neuroexcitatory or neuroinhibitory properties were investigated in the feline vestibular complex. Consecutive semithin sections of plastic-embedded t...
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Article
Profiles enriched in GABA-like immunoreactivity participate in serial synapses in the pontine nuclei of baboon (Papio anubis)
Using a postembedding immunogold procedure with an antiserum against glutaraldehyde-fixed GABA, we demonstrate GABA-like immunoreactivity in two classes of synaptic profiles in the pontine nuclei of baboon. On...
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Article
GABA, glycine, glutamate, aspartate and taurine in the perihypoglossal nuclei: an immunocytochemical investigation in the cat with particular reference to the issue of amino acid colocalization
The differential distribution of glutamate (Glu), aspartate (Asp), glycine (Gly), gamma-aminobutyric acid (GABA) and taurine (Tau) was investigated in the cat's perihypoglossal nuclei. Serial semi-thin (0.5 μm...
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Article
Immunocytochemical evidence suggests that taurine is colocalized with GABA in the Purkinje cell terminals, but that the stellate cell terminals predominantly contain GABA: a light- and electronmicroscopic study of the rat cerebellum
The distributions of taurine-like and GABA-like immunoreactivities in the rat cerebellum were compared by analysis of consecutive semithin and ultrathin sections, postembedding labeled with the peroxidase-anti...
