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Article
Overexpression of GRF-GIF genes enhances plant regeneration in cassava (Manihot esculenta)
The GROWTH-REGULATING FACTOR (GRF) and its INTERACTING FACTOR (GIF) have been shown to stimulate regeneration of transgenic plants with studies reporting increased transformation efficiency in multiple species, i...
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Article
Open AccessMutations in DNA polymerase δ subunit 1 co-segregate with CMD2-type resistance to Cassava Mosaic Geminiviruses
Cassava mosaic disease (CMD) suppresses cassava yields across the tropics. The dominant CMD2 locus confers resistance to cassava mosaic geminiviruses. It has been reported that CMD2-type landraces lose resistance...
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Article
Open AccessAuthor Correction: Biofortification of field-grown cassava by engineering expression of an iron transporter and ferritin
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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Article
Open AccessBiofortification of field-grown cassava by engineering expression of an iron transporter and ferritin
Less than 10% of the estimated average requirement (EAR) for iron and zinc is provided by consumption of storage roots of the staple crop cassava (Manihot esculenta Crantz) in West African human populations. We u...
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Article
Open AccessMultiple morphogenic culture systems cause loss of resistance to cassava mosaic disease
Morphogenic culture systems are central to crop improvement programs that utilize transgenic and genome editing technologies. We previously reported that CMD2-type cassava (Manihot esculenta) cultivars lose resis...
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Article
Open AccessA rapid virus-induced gene silencing (VIGS) method for assessing resistance and susceptibility to cassava mosaic disease
Cassava mosaic disease (CMD) is a major constraint to cassava production in sub-Saharan Africa. Under field conditions, evaluation for resistance to CMD takes 12–18 months, often conducted across multiple year...
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Article
Evaluation of red fluorescent protein (DsRed) as alternative visual marker of genetic transformation in cassava (Manihot esculenta Crantz)
Red fluorescent protein (DsRed) from reef coral was evaluated in comparison with green fluorescent protein (GFP) as a reporter gene for cassava transformation. Cassava friable embryogenic callus (FEC) was tran...
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Article
Open AccessEfficient transmission of Cassava brown streak disease viral pathogens by chip bud grafting
Techniques to study plant viral diseases under controlled growth conditions are required to fully understand their biology and investigate host resistance. Cassava brown streak disease (CBSD) presents a major ...
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Article
Open AccessApplication of FTA technology for sampling, recovery and molecular characterization of viral pathogens and virus-derived transgenes from plant tissues
Plant viral diseases present major constraints to crop production. Effective sampling of the viruses infecting plants is required to facilitate their molecular study and is essential for the development of cro...
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Article
Broad Spectrum Resistance to ssDNA Viruses Associated with Transgene-Induced Gene Silencing in Cassava
Geminiviruses are ssDNA viruses that infect a range of economically important crop species. We have developed a pathogen-derived transgenic approach to generate high levels of resistance against these pathogen...
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Article
Transferring a cassava (Manihot esculenta Crantz) genetic engineering capability to the African environment: Progress and prospects
The procedures required to produce genetically transformed cassava were developed and are now in place in three laboratories in the USA and Europe. Future implementation and sustainability of transgenic techno...
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Article
Production of embryogenic tissues and regeneration of transgenic plants in cassava (Manihot esculenta Crantz)
Disorganised embryogenic tissues have been utilised as target tissues for transgene insertion and transgenic plant regeneration in cassava (Manihot esculenta). The production of friable embryogenic callus in four...
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Article
Expression and inheritance of multiple transgenes in rice plants
The ability to control integration, inheritance, and expression of multiple transgenes is a prerequisite for manipulating biosynthetic pathways and complex agronomic characteristics in plants. One hundred and ...
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Article
Development of friable embryogenic callus and embryogenic suspension culture systems in cassava (Manihot esculenta Crantz)
Procedures for the production of a new and highly prolific embryogenic culture system have been developed in cassava. The importance of the basal salts and type of auxin in controlling the development of cassa...
