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Article
Open AccessImaging Rheumatoid Arthritis in Mice Using Combined Near Infrared and 19F Magnetic Resonance Modalities
Rheumatoid arthritis (RA) is an autoimmune disease that causes pain and tissue destruction in people worldwide. An accurate diagnosis is paramount in order to develop an effective treatment plan. This study de...
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Article
Open AccessConservation of the Amyloid Interactome Across Diverse Fibrillar Structures
Several human proteins cause disease by misfolding and aggregating into amyloid fibril deposits affecting the surrounding tissues. Multiple other proteins co-associate with the diseased deposits but little is ...
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Article
Mild heat treatments induce long-term changes in metabolites associated with energy metabolism in Drosophila melanogaster
Heat-induced hormesis, the beneficial effect of mild heat-induced stress, increases the average lifespan of many organisms. Yet little is known about the mechanisms underlying this effect. We used nuclear magn...
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Article
Open AccessIn situ high-resolution structure of the baseplate antenna complex in Chlorobaculum tepidum
Photosynthetic antenna systems enable organisms harvesting light and transfer the energy to the photosynthetic reaction centre, where the conversion to chemical energy takes place. One of the most complex ante...
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Article
Near-complete 1H, 13C, 15N resonance assignments of dimethylsulfoxide-denatured TGFBIp FAS1-4 A546T
The transforming growth factor beta induced protein (TGFBIp) is a major protein component of the human cornea. Mutations occurring in TGFBIp may cause corneal dystrophies, which ultimately lead to loss of visi...
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Article
Optimized co-solute paramagnetic relaxation enhancement for the rapid NMR analysis of a highly fibrillogenic peptide
Co-solute paramagnetic relaxation enhancement (PRE) is an attractive way to speed up data acquisition in NMR spectroscopy by shortening the T 1 relaxation time of the nucleus of interest and thus...
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Article
Erratum: Hydrogen bond rotations as a uniform structural tool for analyzing protein architecture
Nature Communications 5: Article number: 5803 (2015); Published 17 Dec 2014; Updated 3 Feb 2015. The authors Robert C. Penner and Ebbe S. Andersen were incorrectly omitted from the list of corresponding author...
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Article
Hydrogen bond rotations as a uniform structural tool for analyzing protein architecture
Proteins fold into three-dimensional structures, which determine their diverse functions. The conformation of the backbone of each structure is locally at each Cα effectively described by conformational angles re...
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Article
VirtualSpectrum, a tool for simulating peak list for multi-dimensional NMR spectra
NMR spectroscopy is a widely used technique for characterizing the structure and dynamics of macromolecules. Often large amounts of NMR data are required to characterize the structure of proteins. To save valu...
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Article
Automated robust and accurate assignment of protein resonances for solid state NMR
The process of resonance assignment represents a time-consuming and potentially error-prone bottleneck in structural studies of proteins by solid-state NMR (ssNMR). Software for the automation of this process ...
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Article
Metabolomic analysis of the selection response of Drosophila melanogaster to environmental stress: are there links to gene expression and phenotypic traits?
We investigated the global metabolite response to artificial selection for tolerance to stressful conditions such as cold, heat, starvation, and desiccation, and for longevity in Drosophila melanogaster. Our find...
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Article
Simultaneous acquisition of PAR and PAIN spectra
We present a scheme that allows the simultaneous detection of PAR and PAIN correlation spectra in a single two-dimensional experiment. For both spectra, we obtain almost the same signal-to-noise ratio as if a ...
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Chapter
Dipolar Recoupling
We describe the principles and applications of dipolar recoupling in solid-state NMR spectroscopy as a means to access information about molecular structure and transfer magnetization between spins. In this ma...
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Article
A GC–MS-based metabonomic investigation of blood serum from irritable bowel syndrome patients undergoing intervention with acidified milk products
In the present study, the use of gas chromatography mass spectrometry (GC–MS)-based metabonomics to characterize blood serum in an intervention study of patients suffering from the common gastrointestinal diso...
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Article
A model of the protein–pigment baseplate complex in chlorosomes of photosynthetic green bacteria
In contrast to photosynthetic reaction centers, which share the same structural architecture, more variety is found in the light-harvesting antenna systems of phototrophic organisms. The largest antenna system...
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Article
Open AccessCreatine-induced activation of antioxidative defence in myotube cultures revealed by explorative NMR-based metabonomics and proteomics
Creatine is a key intermediate in energy metabolism and supplementation of creatine has been used for increasing muscle mass, strength and endurance. Creatine supplementation has also been reported to trigger ...
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Article
Cisplatin interaction with phosphatidylserine bilayer studied by solid-state NMR spectroscopy
Cisplatin [cis-diamminedichloridoplatinum(II)] is used in chemotherapy where platinum–DNA adducts initiate tumor cell death. It is possible that side effects such as neurotoxicity and cellular cisplatin resistanc...
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Article
Open AccessThe subunit composition of human extracellular superoxide dismutase (EC-SOD) regulate enzymatic activity
Human extracellular superoxide dismutase (EC-SOD) is a tetrameric metalloenzyme responsible for the removal of superoxide anions from the extracellular space. We have previously shown that the EC-SOD subunit e...
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Chapter
Analytical and Numerical Tools for Experiment Design in Solid-State NMR Spectroscopy
The solid-state NMR spectroscopy research area has evolved tremendously during the past few decades [1–4]. Initially a method of primary interest for physicists, it has become an increasingly powerful and wide...
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Article
Towards high-resolution solid-state NMR on large uniformly 15N- and [13C,15N]-labeled membrane proteins in oriented lipid bilayers
Based on exact numerical simulations, taking into account isotropic and conformation-dependent anisotropic nuclear spin interactions, we systematically analyse the prospects for high-resolution solid-state NMR...
