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Article
Multiple interactions in juxtaposed monolayers of amphibian neuronal, epidermal, and mesodermal limb blastema cells
We have utilized a novel cell culture system to probe nerve-epidermal-blastema cell interactions, in which the cellular participants are plated as compartmentalized juxtaposed monolayers. A mesoderm monolayer ...
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Article
Effect of insulin on cyclic nucleotide levels and promotion of mitosis by insulin and ionophore A23187 in cultured newt blastemata
The levels of cyclic GMP (cGMP) and cyclic AMP (cAMP) were assayed, using radioimmunoassay, in newt blastemata cultured with and without insulin. Our observations show that insulin significantly increases the ...
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Article
Promotion of mitosis in cultured newt limb regenerates by a diffusible nerve factor
Regeneration blastemata of adult newt forelimbs were cultured transfilter to dorsal root ganglia on extremely low porosity (0.05 μm) filters. Mitotic index profiles in these blastemata were compared with those...