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  1. Article

    Open Access

    Structural and functional analysis of tomato sterol C22 desaturase

    Sterols are structural and functional components of eukaryotic cell membranes. Plants produce a complex mixture of sterols, among which β-sitosterol, stigmasterol, campesterol, and cholesterol in some Solanace...

    Laura Gutiérrez-García, Montserrat Arró, Teresa Altabella… in BMC Plant Biology (2021)

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    Farnesyl Diphosphate Synthase Assay

    Farnesyl diphosphate synthase (FPS) catalyzes the sequential head-to-tail condensation of isopentenyl diphosphate (IPP, C5) with dimethylallyl diphosphate (DMAPP, C5) and geranyl diphosphate (GPP, C10) to produce...

    Montserrat Arró, David Manzano, Albert Ferrer in Plant Isoprenoids (2014)

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    Determination of 3-Hydroxy-3-methylglutaryl CoA Reductase Activity in Plants

    The enzyme 3-hydroxy-3-methylglutaryl CoA (HMG-CoA) reductase catalyzes the NADPH-mediated reductive deacylation of HMG-CoA to mevalonic acid, which is the first committed step of the mevalonate pathway for is...

    Narciso Campos, Montserrat Arró, Albert Ferrer, Albert Boronat in Plant Isoprenoids (2014)

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    Article

    Arabidopsis thaliana contains a single gene encoding squalene synthase

    Squalene synthase (SQS) catalyzes the condensation of two molecules of farnesyl diphosphate (FPP) to produce squalene (SQ), the first committed precursor for sterol, brassinosteroid, and triterpene biosynthesis.

    Antoni Busquets, Verónica Keim, Marta Closa, Ana del Arco… in Plant Molecular Biology (2008)

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    Article

    Overexpression of Farnesyl Diphosphate Synthase in Arabidopsis Mitochondria Triggers Light-dependent Lesion Formation and Alters Cytokinin Homeostasis

    To investigate the role of mitochondrial farnesyl diphosphate synthase (FPS) in plant isoprenoid biosynthesis we characterized transgenic Arabidopsis thaliana plants overexpressing FPS1L isoform. This overexpress...

    David Manzano, Antoni Busquets, Marta Closa, Klára Hoyerová… in Plant Molecular Biology (2006)

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    Article

    The metabolic imbalance underlying lesion formation in Arabidopsis thaliana overexpressing farnesyl diphosphate synthase (isoform 1S) leads to oxidative stress and is triggered by the developmental decline of endogenous HMGR activity

    Overexpression of Arabidopsis thaliana farnesyl diphosphate synthase isoform 1S (FPS1S) in transgenic A. thaliana (L.) Heynh. leads to necrotic lesion formation in leaves in planta and to premature senescence in ...

    David Manzano, Xavier Fernández-Busquets, Hubert Schaller, Víctor González… in Planta (2004)

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    Article

    Molecular cloning and expression analysis of the mevalonate kinase gene from Arabidopsis thaliana

    Mevalonate kinase (MVK), the enzyme that catalyzes the phosphorylation of mevalonate to produce mevalonate 5-phosphate, is considered as a potential regulatory enzyme of the isoprenoid biosynthetic pathway. The A...

    Ma Antònia Lluch, Angela Masferrer, Montserrat Arró… in Plant Molecular Biology (2000)