-
Article
A multiplex digital PCR assay for detection and quantitation of porcine circovirus type 2 and type 3
Porcine circovirus (PCV) has become a major pathogen, causing major economic losses in the global pig industry, and PCV type 2 (PCV2) and 3 (PCV3) are distributed worldwide. We designed specific primer and pro...
-
Article
Open AccessThe Double-Deck Viscoelastic Technique: a Novel Surgical Technique to Protect the Corneal Endothelium in Penetrating Keratoplasty of Aphakic Silicone Oil-Dependent Eyes after Severe Ocular Injury
The purpose of this study is to summarize the benefits of the double-deck viscoelastic technique (DDVT), a novel and cost-effective surgical technique that creates a barrier to hinder silicone oil (SO) from co...
-
Article
Probe-based Loop-Mediated Isothermal Amplification Assay for Multi-target Quantitative Detection of Three Foodborne Pathogens in Seafood
Foodborne pathogens pose serious risks to human health and food safety. More than one pathogen exists in seafood. It is difficult for conventional culture methods to detect some foodborne pathogens simultaneou...
-
Article
Open AccessThe Efficacy and Safety of New-Generation Intense Pulsed Light in the Treatment of Meibomian Gland Dysfunction-Related Dry Eye: A Multicenter, Randomized, Patients-Blind, Parallel-Control, Non-Inferiority Clinical Trial
This study aimed to evaluate the efficacy and safety of a new-generation intense pulsed light (IPL) device in improving the symptoms and signs of meibomian gland dysfunction (MGD)-related dry eye, and compare ...
-
Article
Novel immunochromatographic strip assay based on up-conversion nanoparticles for sensitive detection of imidacloprid in agricultural and environmental samples
Neonicotinoid insecticides are widely used in agriculture for pest control, but the pesticide residues in environmental and agricultural products were a big threat to the health of non-target organisms. In thi...
-
Article
Open AccessIn silico analysis of non-synonymous single nucleotide polymorphisms (nsSNPs) in the human GJA3 gene associated with congenital cataract
Gap junction protein alpha 3 (GJA3), an important pathogenic gene of congenital cataracts, encodes the transmembrane protein connexin46, which functions as an intercellular channel for voltage and chemical gating...
-
Article
Open AccessEmploying DNA binding dye to improve detection of Enterocytozoon hepatopenaei in real-time LAMP
Enterocytozoon hepatopenaei (EHP) is a pathogen in the pancreatic tissue of prawn, as reported in recent years. Enterosporidiosis caused by EHP in Penaeus genus is spreading widely, which seriously threatens the ...
-
Article
A simple and efficient method for potential point-of-care diagnosis of human papillomavirus genotypes: combination of isothermal recombinase polymerase amplification with lateral flow dipstick and reverse dot blot
Cervical cancer is the second most common cancer in the world’s woman population with a high incidence in develo** countries where diagnostic conditions for the cancer are poor. The main culprit causing the ...
-
Article
Detection of Viable Vibrio cholerae Cells in Seafood Using a Real-Time Visual Loop-Mediated Isothermal Amplification Combined with Propidium Monoazide
Vibrio cholerae is an important foodborne pathogen causing severe intestinal infectious diseases that have high incidence and mortality. Almost all of rapid testing methods including immu...
-
Article
Open AccessEvaluation of the Effects of Conjunctivochalasis Excision on Tear Stability and Contrast Sensitivity
Conjunctivochalasis (CCh) disrupts tear flow and damages tear film stability. This study sought to evaluate the tear stability and contrast sensitivity of patients with CCh on whom CCh excision was performed. ...
-
Article
Development of a Monoclonal Antibody-Based Immunochromatographic Assay Detecting Ractopamine Residues in Swine Urine
A lateral-flow immunochromatographic assay (LFIA) using monoclonal antibody was developed for the rapid detection of ractopamine residues in swine urine. The assay procedure could be accomplished within 5 min,...
-
Article
Development of Immuno-Based Methods for Detection of Melamine
A competitive indirect enzyme-linked immunosorbent assay (ELISA) and a colloidal gold-based immunochromatographic assay (CGIA) using monoclonal antibodies (mAbs) were developed for the detection of melamine. U...
-
Article
NAT2 slow acetylation genotypes contribute to asthma risk among Caucasians: evidence from 946 cases and 1,091 controls
NAT2 plays a critical role in external chemical detoxification. Thus, polymorphism of NAT2 has been suggested to associate with several disorders. A number of studies have been devoted to the relationship betw...
-
Article
Loop-mediated isothermal amplification (LAMP) method for rapid detection of cry1Ab gene in transgenic rice (Oryza sativa L.)
The cry1Ab gene is a foreign gene which encodes Bt insecticidal Cry1Ab protein and was transferred into genomic DNA of plants to acquire insect resistance. Here a loop-mediated isothermal amplification (LAMP) ass...
-
Article
Development of a loop-mediated isothermal amplification assay for detection of Cronobacter spp. (Enterobacter sakazakii)
Contamination of Cronobacter spp. (Enterobacter sakazakii) in infant formulas and other food products is a severe problem. Here a loop-mediated isothermal amplification (LAMP) assay was developed for rapidly dete...
