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Article
Dynamic simulation on the preparation process of thin films by pulsed laser
An ablation model of targets irradiated by pulsed laser is established. By using the simple energy balance conditions, the relationship between ablation surface location and time is derived. By an adiabatic ap...
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Article
Open AccessModular pathway rewiring of Saccharomyces cerevisiae enables high-level production of L-ornithine
Baker’s yeast Saccharomyces cerevisiae is an attractive cell factory for production of chemicals and biofuels. Many different products have been produced in this cell factory by reconstruction of heterologous bio...
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Article
A cis-eQTL genetic variant of the cancer–testis gene CCDC116 is associated with risk of multiple cancers
Recent studies have found that cancer–testis (CT) genes, which are expressed predominantly in germ and cancer cells, may be candidate cancer drivers. Because of their crucial roles, genetic variants in these g...
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Article
Open AccessEfficient protein production by yeast requires global tuning of metabolism
The biotech industry relies on cell factories for production of pharmaceutical proteins, of which several are among the top-selling medicines. There is, therefore, considerable interest in improving the effici...
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Protocol
High-Throughput Microfluidics for the Screening of Yeast Libraries
Cell factory development is critically important for efficient biological production of chemicals, biofuels, and pharmaceuticals. Many rounds of the Design–Build–Test–Learn cycles may be required before an eng...
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Article
Open AccessBalanced trafficking between the ER and the Golgi apparatus increases protein secretion in yeast
The yeast Saccharomyces cerevisiae is widely used as a cell factory to produce recombinant proteins. However, S. cerevisiae naturally secretes only a few proteins, such as invertase and the mating alpha factor, a...
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Article
Open AccessGlobal rewiring of cellular metabolism renders Saccharomyces cerevisiae Crabtree negative
Saccharomyces cerevisiae is a Crabtree-positive eukaryal model organism. It is believed that the Crabtree effect has evolved as a competition mechanism by allowing for rapid growth and production of ethanol at ae...
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Article
Synthesis of Ni2P/Ni12P5 composite for a highly efficient hydrogen production from formaldehyde solution
A series of nickel phosphide were synthesized by a simple solid phosphidation reaction and used as catalysts for H2 production from formaldehyde solution. It is found that by changing the molar ratio of Ni and P ...
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Article
Identification of A-to-I RNA editing profiles and their clinical relevance in lung adenocarcinoma
Adenosine-to-inosine (A-to-I) RNA editing is a widespread posttranscriptional modification that has been shown to play an important role in tumorigenesis. Here, we evaluated a total of 19,316 RNA editing sites...
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Article
Open AccessImproving recombinant protein production by yeast through genome-scale modeling using proteome constraints
Eukaryotic cells are used as cell factories to produce and secrete multitudes of recombinant pharmaceutical proteins, including several of the current top-selling drugs. Due to the essential role and complexit...
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Article
Open AccessSecretion of collagenases by Saccharomyces cerevisiae for collagen degradation
The production and processing of animal-based products generates many collagen-rich by-products, which have received attention both for exploitation to increase their added value and to reduce their negative e...
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Article
Overexpression of genes by stress-responsive promoters increases protein secretion in Saccharomyces cerevisiae
Recombinant proteins produced by cell factories are now widely used in various fields. Many efforts have been made to improve the secretion capacity of cell factories to meet the increasing demand for recombin...
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Article
Open AccessCorrection: Secretion of collagenases by Saccharomyces cerevisiae for collagen degradation
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Article
Metabolic engineering of yeast for the production of carbohydrate-derived foods and chemicals from C1–3 molecules
The increase in population-related and environmental issues has emphasized the need for more efficient and sustainable production strategies for foods and chemicals. Carbohydrates are macronutrients sourced fr...
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Article
Reproductive outcomes in couples with recurrent pregnancy loss after embryonic chromosomal microarray analysis
Chromosomal microarray analysis (CMA) has been widely applied to explore the genetic etiology in recurrent pregnancy loss (RPL). However, the reproductive prognosis in RPL couples with different types of chrom...
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Article
Advances in recombinant protease production: current state and perspectives
Proteases, enzymes that catalyze the hydrolysis of peptide bonds in proteins, are important in the food industry, biotechnology, and medical fields. With increasing demand for proteases, there is a growing emp...
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Article
Open AccessA PCR-independent approach for mtDNA enrichment and next-generation sequencing: comprehensive evaluation and clinical application
Sequencing the mitochondrial genome has been increasingly important for the investigation of primary mitochondrial diseases (PMD) and mitochondrial genetics. To overcome the limitations originating from PCR-ba...
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Article
Open AccessComprehensive genetic analysis of facioscapulohumeral muscular dystrophy by Nanopore long-read whole-genome sequencing
Facioscapulohumeral muscular dystrophy (FSHD) is a high-prevalence autosomal dominant neuromuscular disease characterized by significant clinical and genetic heterogeneity. Genetic diagnosis of FSHD remains a ...
