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  1. Article

    Open Access

    Endogenous suppression of WNT signalling in human embryonic stem cells leads to low differentiation propensity towards definitive endoderm

    Low differentiation propensity towards a targeted lineage can significantly hamper the utility of individual human pluripotent stem cell (hPSC) lines in biomedical applications. Here, we use monolayer and micr...

    Dominika Dziedzicka, Mukul Tewary, Alexander Keller… in Scientific Reports (2021)

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  2. Article

    Open Access

    Uncovering low-level mosaicism in human embryonic stem cells using high throughput single cell shallow sequencing

    Human pluripotent stem cells (hPSCs) have significant levels of low-grade genetic mosaicism, which commonly used techniques fail to detect in bulk DNA. These copy number variations remain a hurdle for the clin...

    Alexander Keller, Laurentijn Tilleman, Dominika Dziedzicka… in Scientific Reports (2019)

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  3. No Access

    Article

    A High Proliferation Rate is Critical for Reproducible and Standardized Embryoid Body Formation from Laminin-521-Based Human Pluripotent Stem Cell Cultures

    When aiming for homogenous embryoid body (EB) differentiation, the use of equal-sized EBs is required to avoid a size-induced differentiation bias. In this study we developed an efficient and standardized EB f...

    Dominika Dziedzicka, Christina Markouli, Lise Barbé… in Stem Cell Reviews and Reports (2016)

  4. No Access

    Article

    Low-grade chromosomal mosaicism in human somatic and embryonic stem cell populations

    Current knowledge on chromosomal mosaicism in human cell cultures is mostly based on cytogenetic banding methods. The recent development of high-resolution full-genome analysis methods applicable to single cel...

    Kurt Jacobs, Afroditi Mertzanidou, Mieke Geens, Ha Thi Nguyen… in Nature Communications (2014)

  5. No Access

    Article

    Role of BMP Signaling in Pancreatic Progenitor Differentiation from Human Embryonic Stem Cells

    Transplantation of pancreatic progenitors derived from human embryonic stem cells (hESCs) is a promising way to treat diabetes. Strategies to obtain the required cell mass would rely on the up scaling of curre...

    Lina Sui, Mieke Geens, Karen Sermon, Luc Bouwens… in Stem Cell Reviews and Reports (2013)

  6. No Access

    Article

    Human embryonic stem cells commonly display large mitochondrial DNA deletions

    Lindsey Van Haute, Claudia Spits, Mieke Geens, Sara Seneca… in Nature Biotechnology (2013)

  7. No Access

    Protocol

    Establishment of hESC Lines from the Inner Cell Mass of Blastocyst-Stage Embryos and Single Blastomeres of 4-Cell Stage Embryos

    More than 600 human embryonic stem cell (hESC) lines have been reported today at the human European Embryonic Stem Cell Registry (http://www.hescreg.eu/). Despite these ...

    Ileana Mateizel, Mieke Geens, Hilde Van de Velde… in Human Embryonic Stem Cells Handbook (2012)

  8. No Access

    Article

    Sertoli cell-conditioned medium induces germ cell differentiation in human embryonic stem cells

    To investigate the spontaneous germ cell differentiation capacity of VUB hESC lines, develop a protocol for the induction of germ cell differentiation using conditioned medium from Sertoli cells (SCCM) and com...

    Mieke Geens, Karen Dora Sermon… in Journal of Assisted Reproduction and Genet… (2011)

  9. No Access

    Article

    Recurrent chromosomal abnormalities in human embryonic stem cells

    During long-term culture, human embryonic stem (hES) cells may acquire chromosomal abnormalities that compromise their potential clinical utility. A study of 17 hES cell lines reveals various genomic changes, ...

    Claudia Spits, Ileana Mateizel, Mieke Geens, Afroditi Mertzanidou… in Nature Biotechnology (2008)