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    Article

    Identification of Rhodococcus erythropolis Promoters Controlled by Alternative Sigma Factors Using In Vivo and In Vitro Systems and Heterologous RNA Polymerase

    Rhodococcus erythropolis CCM2595 is a bacterial strain, which has been studied for its capability to degrade phenol and other toxic aromatic compounds. Its cell wall contains mycolic acids, which are also an attr...

    Jan Blumenstein, Robert Rädisch, Václav Štěpánek, Michal Grulich in Current Microbiology (2022)

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    Article

    Potential of the strain Raoultella sp. KDF8 for removal of analgesics

    The bacterial strain KDF8 capable of growth in the presence of diclofenac and codeine analgesics was obtained after chemical mutagenesis of nature isolates from polluted soils. The strain KDF8 was identified as R...

    Andrea Palyzová, Jiří Zahradník, Helena Marešová, Lucie Sokolová in Folia Microbiologica (2018)

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    Article

    Potential of Pichia pastoris for the production of industrial penicillin G acylase

    This study deals with the potential of Pichia pastoris X-33 for the production of penicillin G acylase (PGAA) from Achromobacter sp. CCM 4824. Synthetic gene matching the codon usage of P. pastoris was designed f...

    Helena Marešová, Andrea Palyzová, Martina Plačková, Michal Grulich in Folia Microbiologica (2017)

  4. Article

    Erratum to: Penicillin G acylase from Achromobacter sp. CCM 4824

    Stanislav Bečka, Václav Štěpánek in Applied Microbiology and Biotechnology (2014)

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    Article

    Current state and perspectives of penicillin G acylase-based biocatalyses

    In the course of more than 60-year history, penicillin G acylase (PGA) gained a unique position among enzymes used by pharmaceutical industry for production of β-lactam antibiotics. Kinetically controlled enzy...

    Helena Marešová, Martina Plačková, Michal Grulich in Applied Microbiology and Biotechnology (2014)

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    Article

    Penicillin G acylase from Achromobacter sp. CCM 4824

    Penicillin G acylase from Achromobacter sp. (NPGA) was studied in the enzymatic synthesis of β-lactam antibiotics by kinetically controlled N-acylation. When compared with penicillin acylase of Escherichia coli (...

    Stanislav Bečka, Václav Štěpánek in Applied Microbiology and Biotechnology (2014)