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Article
Open AccessSystematic discovery of recombinases for efficient integration of large DNA sequences into the human genome
Large serine recombinases (LSRs) are DNA integrases that facilitate the site-specific integration of mobile genetic elements into bacterial genomes. Only a few LSRs, such as Bxb1 and PhiC31, have been characte...
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Protocol
Single-Molecule Map** of Chromatin Accessibility Using NOMe-seq/dSMF
The bulk of gene expression regulation in most organisms is accomplished through the action of transcription factors (TFs) on cis-regulatory elements (CREs). In eukaryotes, these CREs are generally characterized ...
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Article
Open AccessMassively parallel RNA device engineering in mammalian cells with RNA-Seq
Synthetic RNA-based genetic devices dynamically control a wide range of gene-regulatory processes across diverse cell types. However, the limited throughput of quantitative assays in mammalian cells has hinder...
