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Article
Open AccessStructural basis of promiscuous substrate transport by Organic Cation Transporter 1
Organic Cation Transporter 1 (OCT1) plays a crucial role in hepatic metabolism by mediating the uptake of a range of metabolites and drugs. Genetic variations can alter the efficacy and safety of compounds tra...
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Article
Open AccessBroadly neutralizing SARS-CoV-2 antibodies through epitope-based selection from convalescent patients
Emerging variants of concern (VOCs) are threatening to limit the effectiveness of SARS-CoV-2 monoclonal antibodies and vaccines currently used in clinical practice; broadly neutralizing antibodies and strategi...
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Article
Open AccessChanges within the central stalk of E. coli F1Fo ATP synthase observed after addition of ATP
F1Fo ATP synthase functions as a biological generator and makes a major contribution to cellular energy production. Proton flow generates rotation in the Fo motor that is transferred to the F1 motor to catalyze A...
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Article
Open AccessThe six steps of the complete F1-ATPase rotary catalytic cycle
F1Fo ATP synthase interchanges phosphate transfer energy and proton motive force via a rotary catalysis mechanism. Isolated F1-ATPase catalytic cores can hydrolyze ATP, passing through six intermediate conformati...
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Article
Glutamate transporters have a chloride channel with two hydrophobic gates
Glutamate is the most abundant excitatory neurotransmitter in the central nervous system, and its precise control is vital to maintain normal brain function and to prevent excitotoxicity1. The removal of extracel...
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Article
Open AccessCryo-EM structures provide insight into how E. coli F1Fo ATP synthase accommodates symmetry mismatch
F1Fo ATP synthase functions as a biological rotary generator that makes a major contribution to cellular energy production. It comprises two molecular motors coupled together by a central and a peripheral stalk. ...
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Protocol
ATP Synthase: Expression, Purification, and Function
ATP synthase is an essential enzyme found in all known forms of life, generating the majority of cellular energy via a rotary catalytic mechanism. Here, we describe the in-depth methods for expression, purific...
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Protocol
Rational-Based Protein Engineering: Tips and Tools
The rational engineering of proteins is driven by contemporary needs for new and altered biomolecular forms. Utilizing manipulative procedures of molecular biology, it is relatively straightforward to alter pr...
