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Article
Open AccessCell culture NAIL-MS allows insight into human tRNA and rRNA modification dynamics in vivo
Recently, studies about RNA modification dynamics in human RNAs are among the most controversially discussed. As a main reason, we identified the unavailability of a technique which allows the investigation of...
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Protocol
Quantification of Modified Nucleosides in the Context of NAIL-MS
Recent progress in epitranscriptome research shows an interplay of enzymes modifying RNAs and enzymes dedicated for RNA modification removal. One of the main techniques to study RNA modifications is liquid chr...
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Article
Open AccessTime-resolved NMR monitoring of tRNA maturation
Although the biological importance of post-transcriptional RNA modifications in gene expression is widely appreciated, methods to directly detect their introduction during RNA biosynthesis are rare and do not ...
