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    Antibody-based detection of protein phosphorylation status to track the efficacy of novel therapies using nanogram protein quantities from stem cells and cell lines

    This protocol uses a highly sensitive and reproducible antibody-based capillary isoelectric focusing approach to establish protein phosphorylation status from nanogram quantities of protein cell lysate from ce...

    Mark Aspinall-O'Dea, Andrew Pierce, Francesca Pellicano… in Nature Protocols (2015)