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Protocol
Lysine Bioconjugation on Native Albumin with a Sulfonyl Acrylate Reagent
This protocol details a novel bioconjugation strategy that uses a methanesulfonyl acrylate reagent that is directed to the most reactive lysine on human serum albumin, which enables the construction of chemica...
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Article
Efficient and irreversible antibody–cysteine bioconjugation using carbonylacrylic reagents
There is considerable interest in the development of chemical methods for the precise, site-selective modification of antibodies for therapeutic applications. In this protocol, we describe a strategy for the i...
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Article
Open AccessStoichiometric and irreversible cysteine-selective protein modification using carbonylacrylic reagents
Maleimides remain the reagents of choice for the preparation of therapeutic and imaging protein conjugates despite the known instability of the resulting products that undergo thiol-exchange reactions in vivo. He...
