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Multilayered VBC score predicts sgRNAs that efficiently generate loss-of-function alleles
CRISPR–Cas9 screens have emerged as a transformative approach to systematically probe gene functions. The quality and success of these screens depends on the frequencies of loss-of-function alleles, particular...
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Article
CRISPR-UMI: single-cell lineage tracing of pooled CRISPR–Cas9 screens
Combining each sgRNA with a unique molecular identifier in a genome-wide screen increases sensitivity and robustness in both positive and negative selection.