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Gene structure and expression analyses of multiple vitellogenesis-inhibiting hormones in the whiteleg shrimp Litopenaeus vannamei
In the whiteleg shrimp Litopenaeus vannamei, the existence of six sinus gland peptides (SGPs) possessing vitellogenesis-inhibiting hormone (VIH) function has been previously demonstrated. Genomic and cDNA sequenc...
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White Shrimp Litopenaeus vannamei
The importation of shrimp into Japan at more than 250,000 tons/year is thought to be related in certain aspects to the serious environmental problems that have been caused by shrimp farming in Southeast Asian ...
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Article
Eye structure and function in the giant freshwater prawn Macrobrachium rosenbergii
We used histological and electroretinogram methods to investigate eye structure and function in Macrobrachium rosenbergii. M. rosenbergii was demonstrated to have superposition compound eyes with a clear zone. Rh...
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Article
Alterations of pattern in immune response and vitellogenesis during induced ovarian development by unilateral and bilateral ablation in Litopenaeus vannamei
Eyestalk ablation is often used to induce ovarian development in female shrimp, but it possibly alters the animal’s hormonal balance, and affects its physiology and immune system. Therefore, this study was con...
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Article
Molecular cloning of a cDNA encoding vitellogenesis-inhibiting hormone in the whiteleg shrimp Litopenaeus vannamei and preparation of its recombinant peptide using an E. coli expression system
The cDNA encoding a precursor of Liv-SGP-G, the most abundant crustacean hyperglycemic hormone-family peptide in the sinus gland of the whiteleg shrimp Litopenaeus vannamei, was cloned by RT-PCR coupled with 5′- ...
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Article
The ex vivo effects of eyestalk peptides on ovarian vitellogenin gene expression in the kuruma prawn Marsupenaeus japonicus
Seven major peptides belonging to the crustacean hyperglycemic hormone family were purified from the sinus gland located in the eyestalk of the kuruma prawn Marsupenaeus japonicus, and their effects on vitellogen...
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Article
Dynamics of free amino acids in the hemolymph of Pacific whiteleg shrimp Litopenaeus vannamei exposed to different types of stress
We analyzed the dynamics of amino acids, ammonia-N, and carbohydrates in hemolymph when Pacific whiteleg shrimp Litopenaeus vannamei was subjected to air-exposure and low-salinity stresses. Glycine, l-arginine, a...
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Article
Metabolism of amino acids during hyposmotic adaptation in the whiteleg shrimp, Litopenaeus vannamei
The penaeid prawn, Litopenaeus vannamei, was employed to investigate intracellular isosmotic regulation in situations where invertebrates encounter hyposmosis. Hemolymph osmolality was first analyzed to confirm o...
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Article
Changes in crustacean hyperglycemic hormones in Pacific whiteleg shrimp Litopenaeus vannamei subjected to air-exposure and low-salinity stresses
Changes in crustacean hyperglycemic hormone (CHH)-family peptides in response to stress were investigated in Litopenaeus vannamei. Stress treatments consisted of air exposure and low salinity. High-performance li...
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Article
Molecular cloning and characterization of Dmc1, a gene involved in gametogenesis, from the whiteleg shrimp Litopenaeus vannamei
In order to gain a better understanding of the developmental processes of shrimp germ cells, the molecular cloning and characterization of a meiosis-related gene, Dmc1, was carried out using the whiteleg shrimp L...
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Article
Difference in light perception capability and spectral response between juveniles and sub-adults of the whiteleg shrimp Litopenaeus vannamei as determined by electroretinogram
In this study, we determined light perception capability in juveniles and sub-adults of L. vannamei by means of electroretinogram (ERG) measurements. Eyes were assumed to be dark-adapted w...
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Article
Carbonic anhydrase and Na/K-ATPase activities during the molt cycle of low salinity-reared white shrimp Litopenaeus vannamei
Changes in hemolymph osmolality, ion concentrations, and enzymatic activities of carbonic anhydrase (CA) in the gills and epidermal tissue, and Na/K-ATPase in the gills during the molt cycle were investigated ...
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Article
Carbonic anhydrase and Na/K-ATPase activities at different molting stages of the giant freshwater prawn Macrobrachium rosenbergii
In the present study, the role of carbonic anhydrase (CA) and Na/K-ATPase in the gill and epidermal tissues in the giant freshwater prawn Macrobrachium rosenbergii was examined as a function of molting stage. CA ...
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Article
Purification of Sinus Gland Peptides Having Vitellogenesis-Inhibiting Activity from the Whiteleg Shrimp Litopenaeus Vannamei
Vitellogenesis-inhibiting hormone (VIH) in Crustacea belongs to the crustacean hyperglycemic hormone (CHH)-family. To characterize multiple VIH molecules in the whiteleg shrimp Litopenaeus vannamei, seven CHH-...
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Article
Current status of freshwater prawn culture in Vietnam and the development and transfer of seed production technology
In Vietnam, the giant freshwater prawn Macrobrachium rosenbergii is becoming an increasingly important targeted species, as its culture, especially in rice fields, is considered to have the potential to raise inc...
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Article
The effects of dietary lipid sources and lecithin on the production of giant freshwater prawn Macrobrachium rosenbergii larvae in the Mekong Delta region of Vietnam
The giant freshwater prawn, Macrobrachium rosenbergii, is cultured widely in the Mekong Delta region of Vietnam but it is often difficult or expensive for hatchery operators to purchase commercial diets used as a...
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Article
The dynamics of vitellogenin gene expression differs between intact and eyestalk ablated kuruma prawn Penaeus (Marsupenaeus) japonicus
In order to compare the dynamics of vitellogenin gene expression between naturally maturing prawns and prawns induced to mature artificially by eyestalk ablation, a cDNA encoding vitellogenin was cloned from a...