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Article
Efficient somatic embryogenesis in sugar beet (Beta vulgaris L.) breeding lines
Efficient regeneration via somatic embryogenesis (SE) would be a valuable system for the micropropagation and genetic transformation of sugar beet. This study evaluated the effects of basic culture media (MS a...
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Article
Efficient procedures for callus induction and adventitious shoot organogenesis in sugar beet (Beta vulgaris L.) breeding lines
Improved in vitro tissue culture systems are needed to facilitate the application of transgene technology to the improvement of sugar beet germplasms. Several commercially important sugar beet breeding lines (SDM...
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Chapter
Arabidopsis CDC2a and Cyclin Gene Promoter::gusA Constructs as Markers of Cell Growth and Division in Heterologous Plants
Cell growth and division are integrated with a programme of differentiation to determine the form of the plant body. In recent years the molecular controls of cell division and growth have been elucidated. Pro...
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Article
Manipulation of discriminatory T-DNA delivery by Agrobacterium into cells of immature embryos of barley and wheat
Immature embryos (IEs) of barley (Hordeum vulgare L.) and wheat (Triticum aestivum L.) were co-cultivated with Agrobacterium tumefaciens and the transfer of T-DNA to the cereals was assessed by analysis of the ex...
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Article
Efficient Co-Transformation of Nicotiana Tabacum by two Independent T-DNAs, the Effect of T-DNA Size and Implications for Genetic Separation
The co-transformation of a single plant genome with two independent T-DNA regions provides opportunities for genetic separation in subsequent generations. In an effective strategy, co-delivery events must form...
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Article
Thidiazuron-induced organogenesis and somatic embryogenesis in sugar beet (Beta vulgaris L.)
Improved in vitro tissue culture systems are needed to facilitate the application of recombinant DNA technology to the improvement of sugar beet germplasm. The effects of N 6-benzyladenine...
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Article
Book Reviews
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Article
Agrobacterium-mediated stable transformation of cell suspension cultures of barley (Hordeum vulgare)
Barley (Hordeum vulgare L. cvs. Igri and Dissa) cell suspension cultures, which had been initiated from immature embryo-derived (IED) and microspore-derived (MSD) callus, were co-cultivated with various Agroba...
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Article
The use of visual marker genes as cell-specific reporters of Agrobacterium-mediated T-DNA delivery to wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.).
Transfer of T-DNA from Agrobacterium tumefaciens and A. rhizogenes to cells of wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.) is demonstrated following the inoculation of immature embryos and imm...
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Article
Agrobacterium-mediated transformation of élite indica and japonica rice cultivars
A rapid, efficient, routine system has been established forAgrobacterium tumefaciens-mediated production of hundreds of fertile transgenic plants from commercially important rice cultivars, including an indica cu...
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Article
Tryptophan and indole-3-acetic acid accumulation in Acer cell cultures and its relationship with cell autolysis
The accumulation and decline of ‘free indole-3-acetic acid (IAA)’ and tryptophan has been monitored in cells of Acer pseudoplatanus L. grown in batch suspension cultures. The period of maximal IAA accumulation pe...