6 Result(s)
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Article
Disentangling of Information About the Structure of Biomolecules Based on the Decomposition and Separation of Two-Dimentional Charge Distributions of Ions
Electrospray ionization mass spectra of biomolecules typically consist of a series of multiply charged ions because of the transfer of protons or other charge carriers between ions of biomolecules and the surr...
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Article
Predictive Liquid Chromatography of Peptides Based on Hydrophilic Interactions for Mass Spectrometry-Based Proteomics
High-performance liquid chromatography (HPLC) is widely used for separation of complex peptide mixtures before mass spectrometry-based proteome analysis. In this analysis, reversed phase HPLC (RPHPLC) using no...
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Article
Applicability of the critical chromatography concept to proteomic problems. II. Effect of mobile phase on the separation of peptides and proteins taking into account the amino acid sequence
The effect of parameters of a binary mobile phase (chemical properties of components, gradient profile, flow rate, and pH) on the separation of peptides and proteins is theoretically investigated using a model...
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Article
Applicability of the critical chromatography concept to proteomics problems: I. Effect of the stationary phase and the size of the chromatographic column on the dependence of the retention time of peptides and proteins on the amino acid sequence
A theoretical study of the effect of stationary phase parameters on the regularities of the separation of peptides and proteins using a model of critical chromatography BioLCCC and the Theoretical chromatography ...
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Article
Peptide identification in “shotgun” proteomics using tandem mass spectrometry: Comparison of search engine algorithms
High-throughput proteomics technologies are gaining popularity in different areas of life sciences. One of the main objectives of proteomics is characterization of the proteins in biological samples using liqu...
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Article
Inversion of chromatographic elution orders of peptides and its importance for proteomics
Inversion of the order of peptide elution in reversed-phase liquid chromatography under changing separation conditions, such as gradient slope has been considered. Using a six-protein proteolytic peptide stand...
