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Article
Aberrant activation of p53/p66Shc-mInsc axis increases asymmetric divisions and attenuates proliferation of aged mammary stem cells
Aging is accompanied by the progressive decline in tissue regenerative capacity and functions of resident stem cells (SCs). Underlying mechanisms, however, remain unclear. Here we show that, during chronologic...
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Article
Open AccessXenoCell: classification of cellular barcodes in single cell experiments from xenograft samples
Single-cell sequencing technologies provide unprecedented opportunities to deconvolve the genomic, transcriptomic or epigenomic heterogeneity of complex biological systems. Its application in samples from xeno...
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Article
Release of paused RNA polymerase II at specific loci favors DNA double-strand-break formation and promotes cancer translocations
It is not clear how spontaneous DNA double-strand breaks (DSBs) form and are processed in normal cells, and whether they predispose to cancer-associated translocations. We show that DSBs in normal mammary cell...
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Article
Open AccessEpigenomic profiling of archived FFPE tissues by enhanced PAT-ChIP (EPAT-ChIP) technology
The introduction of pathology tissue-chromatin immunoprecipitation (PAT-ChIP), a technique allowing chromatin immunoprecipitation (ChIP) from formalin-fixed paraffin-embedded (FFPE) tissues, has extended the a...
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Article
Open AccessPREP1 tumor suppressor protects the late-replicating DNA by controlling its replication timing and symmetry
The synthesis of middle-to-late-replicating DNA can be affected independently of the rest of the genome by down-regulating the tumor suppressor PREP1 (PKNOX1). Indeed, DNA combing shows that PREP1 down-regulat...
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Article
Open AccessA knowledge-based framework for the discovery of cancer-predisposing variants using large-scale sequencing breast cancer data
The landscape of cancer-predisposing genes has been extensively investigated in the last 30 years with various methodologies ranging from candidate gene to genome-wide association studies. However, sequencing ...
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Article
Myc-binding-site recognition in the human genome is determined by chromatin context
Large-scale chromatin immunoprecipitation (ChIP) studies have been effective in unravelling the distribution of DNA-binding transcription factors along eukaryotic genomes1, but specificity determinants remain elu...
