23 Result(s)

Protocol

Fluorescence Anisotropy and Polarization in the Characterization of Biomolecular Association Processes and Their Application to Study SH2 Domain Binding Affinity

Fluorescence anisotropy (or polarization) is a powerful technique to study biomolecular association processes, by following the rotational motions of one of the two partners in the interaction, labeled with a ...

Sara Bobone, Claudia Storti, Paolo Calligari, Lorenzo Stella in SH2 Domains (2023)

Protocol

Computational Evaluation of Peptide–Protein Binding Affinities: Application of Potential of Mean Force Calculations to SH2 Domains

Many biological functions are mediated by protein–protein interactions (PPIs), often involving specific structural modules, such as SH2 domains. Inhibition of PPIs is a pharmaceutical strategy of growing impor...

Paolo Calligari, Lorenzo Stella, Gianfranco Bocchinfuso in SH2 Domains (2023)

Article

Douglas–Rachford splitting and ADMM for nonconvex optimization: accelerated and Newton-type linesearch algorithms

Although the performance of popular optimization algorithms such as the Douglas–Rachford splitting (DRS) and the ADMM is satisfactory in convex and well-scaled problems, ill conditioning and nonconvexity pose ...

Andreas Themelis, Lorenzo Stella… in Computational Optimization and Applications (2022)

Chapter and Conference Paper

Anomaly Detection at Scale: The Case for Deep Distributional Time Series Models

This paper introduces a new methodology for detecting anomalies in time series data, with a primary application to monitoring the health of (micro-) services and cloud resources. The main novelty in our approa...

Fadhel Ayed, Lorenzo Stella… in Service-Oriented Computing – ICSOC 2020 W… (2021)

Chapter

Selectivity of Antimicrobial Peptides: A Complex Interplay of Multiple Equilibria

Antimicrobial peptides (AMPs) attack bacterial membranes selectively, killing microbes at concentrations that cause no toxicity to the host cells. This selectivity is not due to interaction with specific recep...

Sara Bobone, Lorenzo Stella in Antimicrobial Peptides (2019)

Article

Forward–backward quasi-Newton methods for nonsmooth optimization problems

The forward–backward splitting method (FBS) for minimizing a nonsmooth composite function can be interpreted as a (variable-metric) gradient method over a continuously differentiable function which we call for...

Lorenzo Stella, Andreas Themelis… in Computational Optimization and Applications (2017)

Article

Mutations in KCNH1 and ATP6V1B2 cause Zimmermann-Laband syndrome

Kerstin Kutsche, Marco Tartaglia and colleagues show that missense mutations in KCNH1 and ATP6V1B2 cause Zimmermann-Laband syndrome, a disorder characterized by facial dysmorphism, intellectual disability, digit ...

Fanny Kortüm, Viviana Caputo, Christiane K Bauer, Lorenzo Stella… in Nature Genetics (2015)

Article

Mutations in ZBTB20 cause Primrose syndrome

Marco Tartaglia, Raoul Hennekam and colleagues show that de novo mutations in ZBTB20 cause Primrose syndrome, a disorder characterized by tall stature, macrocephaly, intellectual disability, diabetes, deafness, p...

Viviana Cordeddu, Bert Redeker, Emilia Stellacci, Aldo Jongejan… in Nature Genetics (2014)

Article

Yves Mély and Guy Duportail (Eds.): Fluorescent methods to study biological membranes

Lorenzo Stella in Analytical and Bioanalytical Chemistry (2013)

Article

Evidences of a natively unfolded state for the human topoisomerase IB N-terminal domain

The N-terminal domain of human topoisomerase IB has been expressed, purified and characterized by spectroscopic techniques. CD spectra as a function of concentration and pH indicate that the domain does not po...

Oscar Vassallo, Silvia Castelli, Ilda D’Annessa, Blasco Morozzo della Rocca… in Amino Acids (2011)

Article

Fluorescence spectroscopy and molecular dynamics simulations in studies on the mechanism of membrane destabilization by antimicrobial peptides

Since their initial discovery, 30 years ago, antimicrobial peptides (AMPs) have been intensely investigated as a possible solution to the increasing problem of drug-resistant bacteria. The interaction of antim...

Gianfranco Bocchinfuso, Sara Bobone… in Cellular and Molecular Life Sciences (2011)

Chapter

Peptide Foldamers: From Spectroscopic Studies to Applications

Peptide foldamers are synthetic oligopeptides which attain a few, specific, constrained conformations in solution. Here, we review our contributions to the study of the structural features of several foldamers...

Lorenzo Stella, Gianfranco Bocchinfuso, Emanuela Gatto… in Reviews in Fluorescence 2008 (2010)

Chapter and Conference Paper

Monitoring Peptide Folding by Time-Resolved Spectroscopies: the Effect of a Single Gly to Aib Susbtitution

Mariano Venanzi, Emanuela Gatto, Gianfranco Bocchinfuso… in Peptides for Youth (2009)

Chapter and Conference Paper

Antimicrobial Peptides Chelating Lanthanide Ions: the Case of Trichogin GA IV Analogues and Terbium(III)

Mariano Venanzi, Emanuela Gatto, Lorenzo Stella… in Peptides for Youth (2009)

Chapter and Conference Paper

Alamethicin Interaction with Lipid Membranes: A Spectroscopic Study on Synthetic Analogs

Lorenzo Stella, Marcello Burattini, Claudia Mazzuca… in Understanding Biology Using Peptides (2006)

Chapter and Conference Paper

A Time-Resolved Spectroscopic Study on Peptide Folding

Basilio Pispisa, Emanuela Gatto… in Understanding Biology Using Peptides (2006)

Chapter and Conference Paper

Intramolecular Triplet Quenching by Nitroxide Radicals as a Tool for Determining Peptide Secondary Structure in Solution

Lorenzo Stella, Gianfranco Bocchinfuso… in Understanding Biology Using Peptides (2006)