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Article
Non-structural proteins of bovine viral diarrhea virus
Bovine viral diarrhea virus (BVDV) belongs to the family Flaviviridae genus pestivirus. The viral genome is a single-stranded, positive-sense RNA that encodes four structural proteins (i.e., C, Erns, E1, and E...
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Article
Open AccessBrucella melitensis UGPase inhibits the activation of NF-κB by modulating the ubiquitination of NEMO
UTP-glucose-1-phosphoryl transferase (UGPase) catalyzes the synthesis of UDP-glucose, which is essential for generating the glycogen needed for the synthesis of bacterial lipopolysaccharide (LPS) and capsular ...
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Article
Open AccessHuman cells are permissive for the productive infection of porcine circovirus type 2 in vitro
Porcine circovirus 2 (PCV2) is the main pathogen of porcine circovirus diseases and porcine circovirus-associated diseases, which are widespread in swine-producing countries. However, there is controversy rega...
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Article
Mink Circovirus Can Infect Minks, Foxes and Raccoon Dogs
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Article
Open AccessImmunogenicity evaluation of inactivated virus and purified proteins of porcine circovirus type 2 in mice
Vaccination is considered as an effective and economical way to against PCV2 infection. However, some of commercial available vaccines are based on inactivated viruses, while the others are based on purified p...
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Article
Interactions of porcine circovirus 2 with its hosts
Porcine circovirus 2 (PCV2) can cause porcine circovirus diseases and porcine circovirus-associated diseases (PCVD/PCVAD), which are widely presented in swine-producing countries. Since the discovery of this v...
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Article
Live Cell Reporter Systems for Positive-Sense Single Strand RNA Viruses
Cell-based reporter systems have facilitated studies of viral replication and pathogenesis, virus detection, and drug susceptibility testing. There are three types of cell-based reporter systems that express c...
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Article
Development of a Rapid Method for the Visible Detection of Pork DNA in Halal Products by Loop-Mediated Isothermal Amplification
Disclosing the composition of meat products in detail is essential to consumers’ rights. In this study, we developed a rapid method based on loop-mediated isothermal amplification to visibly identify pork DNA ...
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Article
Markerless Deletion System for Escherichia coli Using Short Homologous Sequences and Positive–Negative Selectable Cassette
Red homologous recombination has been extensively used in recombineering. Because foreign sequences, such as antibiotic resistance genes, FRT-sites, or loxP-sites, are often unwanted in mutant Escherichia coli, w...
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Article
Analysis of molecular variation in porcine reproductive and respiratory syndrome virus in China between 2007 and 2012
In the present study, 89 porcine reproductive and respiratory syndrome virus (PRRSV) isolates in China during 2007 to 2012 were randomly selected from the GenBank genetic sequence database. Evolutionary charac...
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Article
Porcine CD4 promoters and enhancers can direct foreign gene expression in human cells
Porcine CD4 proximal promoter and enhancer sequences were cloned and aligned with the corresponding human and murine sequences. The alignment showed nucleotide homology between porcine and human sequences was ...
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Article
Construction of a recombinant human FGF1 expression vector for mammary gland-specific expression in human breast cancer cells
Human Fibroblast growth factor 1 (FGF1) has been recognized as a valuable protein drug for the treatment of many diseases because of its multiple functions in regulating a variety of biological processes invol...
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Article
Comparative Proteomic Analyses of Streptococcus suis Serotype 2 Cell Wall-Associated Proteins
Streptococcus suis serotype 2 (SS2) is a zoonotic pathogen that is distributed throughout the world. Virulence factors and/or markers of the virulent serotype 2 strains have not been fully identified. In this stu...
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Article
Open AccessMolecular characterization of a Chinese variant of the Flury-LEP strain
The entire genome of rabies virus vaccine strain Flury-LEP-C, a Chinese variant of the rabies virus vaccine strain Flury-LEP, was sequenced. The overall length of the genome of Flury-LEP-C strain was 11 924 nu...
