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  1. No Access

    Protocol

    Conjugation Site Analysis by MS/MS Protein Sequencing

    In-depth knowledge about the site of drug-linker conjugation is important for the understanding of the conjugation efficiency and the exact locations of payloads for antibody-drug conjugates (ADCs). Here we de...

    Linjie Han, Yanqun Zhao, Qunying Zhang in Antibody-Drug Conjugates (2020)

  2. No Access

    Article

    Analysis of a Soluble (UreD:UreF:UreG)2 Accessory Protein Complex and Its Interactions with Klebsiella aerogenes Urease by Mass Spectrometry

    Maturation of the nickel-containing urease of Klebsiella aerogenes is facilitated by the UreD, UreF, and UreG accessory proteins along with the UreE metallo-chaperone. A fusion of the maltose binding protein and ...

    Mark A. Farrugia, Linjie Han, Yueyang Zhong… in Journal of The American Society for Mass S… (2013)

  3. No Access

    Article

    Traveling-wave ion mobility-mass spectrometry reveals additional mechanistic details in the stabilization of protein complex ions through tuned salt additives

    Ion mobility–mass spectrometry is often applied to the structural elucidation of multiprotein assemblies in cases where X-ray crystallography or NMR experiments have proved challenging. Such applications are g...

    Linjie Han, Brandon T. Ruotolo in International Journal for Ion Mobility Spectrometry (2013)