12 Result(s)
within
Liji **e
Biomedicine
Virology
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Article
Development of a real-time multiplex PCR assay for detection of viral pathogens of penaeid shrimp
A real-time multiplex polymerase chain reaction (rtm-PCR) assay was developed and optimized to simultaneously detect three viral pathogens of shrimp in one reaction. Three sets of specific oligonucleotide prim...
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Article
Open AccessVisual detection of H3 subtype avian influenza viruses by reverse transcription loop-mediated isothermal amplification assay
Recent epidemiological investigation of different HA subtypes of avian influenza viruses (AIVs) shows that the H3 subtype is the most predominant among low pathogenic AIVs (LPAIVs), and the seasonal variations...
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Article
Sequencing and phylogenetic analysis of an avian reovirus genome
Avian reovirus infection causes considerable economic loss to the commercial poultry industry. Live-attenuated vaccine strain S1133 (v-S1133, derived from parent strain S1133) is considered the safest and most...
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Article
Open AccessA loop-mediated isothermal amplification assay for the visual detection of duck circovirus
Duck circovirus (DuCV) infection in farmed ducks is associated with growth problems or retardation syndromes. Rapid identification of DuCV infected ducks is essential to control DuCV effectively. Therefore, this ...
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Article
Role of phosphatidylinositol 3-kinase (PI3K) and Akt1 kinase in porcine reproductive and respiratory syndrome virus (PRRSV) replication
We have previously reported that inhibition of phosphatidylinositol 3-kinase (PI3K) reduces porcine reproductive and respiratory syndrome (PRRSV) replication. Here, we further investigate the mechanism by whic...
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Article
Complete genomic sequence of the Vibrio alginolyticus lytic bacteriophage PVA1
A novel Vibrio alginolyticus lytic bacteriophage was isolated from sewage samples obtained from a local aquatic market. Morphological analysis revealed that the phage, designated as PVA1, belonged to the family P...
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Article
Open AccessA duplex real-time PCR assay for the detection and quantification of avian reovirus and Mycoplasma synoviae
Infectious arthritis in broilers represents an economic and health problem, resulting in severe losses due to retarded growth and downgrading at the slaughterhouse. The most common agents associated with cases...
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Open AccessReverse-transcription, loop-mediated isothermal amplification assay for the sensitive and rapid detection of H10 subtype avian influenza viruses
The H10 subtype avian influenza viruses (H10N4, H10N5 and H10N7) have been reported to cause disease in mammals, and the first human case of H10N8 subtype avian influenza virus was reported in 2013. Recently, ...
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Article
Open AccessSimultaneous detection of eight immunosuppressive chicken viruses using a GeXP analyser-based multiplex PCR assay
Immunosuppressive viruses are frequently found as co-infections in the chicken industry, potentially causing serious economic losses. Because traditional molecular biology methods have limited detection abilit...
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Article
Avian reovirus σA and σNS proteins activate the phosphatidylinositol 3-kinase-dependent Akt signalling pathway
The present study was conducted to identify avian reovirus (ARV) proteins that can activate the phosphatidylinositol 3-kinase (PI3K)-dependent Akt pathway. Based on ARV protein amino acid sequence analysis, σA...
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Article
Genetic and phylogenetic analysis of a novel parvovirus isolated from chickens in Guangxi, China
A previously unidentified chicken parvovirus (ChPV) strain, associated with runting-stunting syndrome (RSS), is now endemic among chickens in China. To explore the genetic diversity of ChPV strains, we determi...
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Article
Altered gene expression profiles of the MDA5 signaling pathway in peripheral blood lymphocytes of chickens infected with avian reovirus
Avian reovirus (ARV) is a member of the genus Orthoreovirus in the family Reoviridae and causes a severe syndrome including viral arthritis that leads to considerable losses in the poultry industry. Innate immuni...
