26 Result(s)

Article

Insights into pollen–stigma recognition: self-incompatibility mechanisms serve as interspecies barriers in Brassicaceae?

A new study provides a comprehensive molecular mechanism that controls interspecific incompatibility of self-incompatible (SI) plants in the Brassicaceae. This finding points to a potentially promising path to...

Zijun Lan, Sheng Zhong, Li-Jia Qu in aBIOTECH (2023)

Article

Distinct chromatin signatures in the Arabidopsis male gametophyte

Epigenetic reprogramming in the germline contributes to the erasure of epigenetic inheritance across generations in mammals but remains poorly characterized in plants. Here we profiled histone modifications th...

Danling Zhu, Yi Wen, Wanyue Yao, Haiyan Zheng, Sixian Zhou, Qiqi Zhang… in Nature Genetics (2023)

Protocol

Obtaining Mutant Pollen for Phenotypic Analysis and Pollen Tube Dual Staining

Mutant phenotype observation is the most useful and important method to study which biological process a gene-of-interest is involved in. In flowering plants, excessive pollen grains land and germinate on the ...

Sheng Zhong, Zhijuan Wang, Li-Jia Qu in Pollen and Pollen Tube Biology (2020)

Protocol

Semi-In Vivo Assay for Pollen Tube Attraction

In flowering plants, each pollen tube delivers two sperm cells into the ovule to complete double fertilization. During the process, pollen tubes need to be navigated into the ovule, where accurate and complex ...

Sheng Zhong, Zhijuan Wang, Li-Jia Qu in Pollen and Pollen Tube Biology (2020)

Article

Cysteine-rich peptides: signals for pollen tube guidance, species isolation and beyond

Sheng Zhong, Li-Jia Qu in Science China Life Sciences (2019)

Article

Sperm cells are passive cargo of the pollen tube in plant fertilization

Sperm cells of seed plants have lost their motility and are transported by the vegetative pollen tube cell for fertilization, but the extent to which they regulate their own transportation is a long-standing d...

Jun Zhang, Qingpei Huang, Sheng Zhong, Andrea Bleckmann, Jiaying Huang… in Nature Plants (2017)

Protocol

Generation and Identification of Arabidopsis EMS Mutants

EMS mutant analysis is a routine experiment to identify new players in a specific biological process or signaling pathway using forward genetics. It begins with the generation of mutants by treating Arabidopsis s...

Li-Jia Qu, Genji Qin in Arabidopsis Protocols (2014)

Protocol

Generation and Characterization of Arabidopsis T-DNA Insertion Mutants

Transfer DNA (T-DNA) insertion mutants are often used in forward and reverse genetics to reveal the molecular mechanisms of a particular biological process in plants. To generate T-DNA insertion mutants, T-DNA...

Li-Jia Qu, Genji Qin in Arabidopsis Protocols (2014)

Article

Functional conservation of the plant EMBRYONIC FLOWER2 gene between bamboo and Arabidopsis

A novel EMBRYONGIC FLOWER2 gene, DlEMF2, was isolated from bamboo, Dendrocalamus latiflorus. It is 2518 bp in length encoding a protein of 629 amino acids. It was expressed in all organs and at a higher level in ...

Hong Xu, Ling-**g Chen, Li-Jia Qu, Hong-Ya Gu, De-Zhu Li in Biotechnology Letters (2010)

Article

Involvement of an R2R3-MYB transcription factor gene AtMYB118 in embryogenesis in Arabidopsis

MYB transcription factors play important roles in various developmental processes in plants. Here we report the characterization of AtMYB118, a gene encoding a putative R2R3-type MYB transcription factor, which e...

Yunfei Zhang, Guangyu Cao, Li-Jia Qu, Hongya Gu in Plant Cell Reports (2009)

Article

The possible action mechanisms of indole-3-acetic acid methyl ester in Arabidopsis

We previously reported that Arabidopsis indole-3-acetic acid (IAA)-methyltransferase-1 (IAMT1) catalyzes the conversion of IAA, an essential phytohormone, to methyl-IAA (MeIAA) and that IAMT1 plays an important r...

Linchuan Li, **anhui Hou, Tomohiko Tsuge, Maoyu Ding, Takashi Aoyama… in Plant Cell Reports (2008)

Article

A gain-of-function mutation of transcriptional factor PTL results in curly leaves, dwarfism and male sterility by affecting auxin homeostasis

GT factors are plant-specific trihelix DNA-binding transcription factors, which are involved in light responses and other developmental processes in plant. We identified a gain-of-function mutant of a GT-2 fac...

**n Li, Genji Qin, Zhangliang Chen, Hongya Gu, Li-Jia Qu in Plant Molecular Biology (2008)

Article

Identification and characterization of COI1-dependent transcription factor genes involved in JA-mediated response to wounding in Arabidopsis plants

The phytohormone jasmonic acid (JA) is an important signaling molecular involved in many developmental and physiological processes, especially in the response of plants to wounding. In this study, we adopted a...

Zhe Wang, Guangyu Cao, **nlei Wang, ** Miao, **aoting Liu… in Plant Cell Reports (2008)