16 Result(s)
within
Li-**n He
Biotechnology
Environmental Engineering/Biotechnology
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Article
Open AccessMetabolic engineering of Escherichia coli for direct production of vitamin C from D-glucose
Production of vitamin C has been traditionally based on the Reichstein process and the two-step process. However, the two processes share a common disadvantage: vitamin C cannot be directly synthesized from D-...
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Article
Open AccessBiochar-mediated enhanced ethanol fermentation (BMEEF) in Zymomonas mobilis under furfural and acetic acid stress
Pretreatment of lignocellulosic biomass generates different types of inhibitors (e.g., furfural and acetic acid), which could remarkably inhibit subsequent ethanol fermentation. Here, biochar as an additive in...
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Article
Open AccessReplacing process water and nitrogen sources with biogas slurry during cellulosic ethanol production
Environmental issues, such as the fossil energy crisis, have resulted in increased public attention to use bioethanol as an alternative renewable energy. For ethanol production, water and nutrient consumption ...
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Article
Open AccessComparative genomic, transcriptomic and secretomic profiling of Penicillium oxalicum HP7-1 and its cellulase and xylanase hyper-producing mutant EU2106, and identification of two novel regulatory genes of cellulase and xylanase gene expression
The filamentous fungus Penicillium oxalicum is a potential alternative to Trichoderma reesei for industrial production of a complete cellulolytic enzyme system for a bio-refinery. Comparative omics approaches can...
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Article
A novel process for obtaining pinosylvin using combinatorial bioengineering in Escherichia coli
Pinosylvin as a bioactive stilbene is of great interest for food supplements and pharmaceuticals development. In comparison to conventional extraction of pinosylvin from plant sources, biosynthesis engineering...
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Article
Increased polysaccharide production and biosynthetic gene expressions in a submerged culture of Ganoderma lucidum by the overexpression of the homologous α-phosphoglucomutase gene
This study aimed to improve the production of polysaccharide by engineering the biosynthetic pathway in Ganoderma lucidum through the overexpression of α-phosphoglucomutase (PGM) gene. PGM is responsible for the...
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Article
Regulation of docosahexaenoic acid production by Schizochytrium sp.: effect of nitrogen addition
Docosahexaenoic acid (DHA) percentage in total fatty acids (TFAs) is an important index in DHA microbial production. In this study, the change of DHA percentage in response to fermentation stages and the strat...
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Article
Optimization of fermentation process for the production of intracellular polysaccharide from Paecilomyces cicadae and the immuno-stimulating activity of intracellular polysaccharide
Optimization of fermentation process for the production of intracellular polysaccharide (IPS) from the fungus Paecilomyces cicadae and the immuno-stimulating activity of IPS were carried out. The quantitative eff...
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Article
Catalytic characteristics of plant-esterase from wheat flour
A plant-esterase extracted from wheat flour and purified with a PEG1000/NaH2PO4 aqueous two-phase system was characterized for its catalytic characteristics. The optimal condition for plant-esterase to catalyze 1...
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Article
Cloning and biochemical properties of a highly thermostable and enantioselective nitrilase from Alcaligenes sp. ECU0401 and its potential for (R)-(−)-mandelic acid production
A nitrilase gene from Alcaligenes sp. ECU0401 was cloned and overexpressed in Escherichia coli BL21 (DE3) in a soluble form. The encoded protein with a His6-tag was purified to nearly homogeneity as revealed by S...
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Article
Strain improvement of Serratia marcescens ECU1010 and medium cost reduction for economic production of lipase
Industrial strain improvement plays a central role in the commercial development of microbial fermentation processes. The strain of Serratia marcescens ECU1010, a wild-type lipase-producer capable of stereospecif...
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Article
Enhanced docosahexaenoic acid production by reinforcing acetyl-CoA and NADPH supply in Schizochytrium sp. HX-308
Docosahexaenoic acid (DHA) production in Schizochytrium sp. HX-308 was evaluated by detecting enzymatic activities of ATP:citrate lyase (EC 4.1.3.8), malic enzyme (EC 1.1.1.40) and glucose-6-phosphate dehydrogena...
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Article
Lipase-catalyzed transesterification of soybean oil for biodiesel production in tert-amyl alcohol
Lipase-catalyzed transesterification of soybean oil and methanol for biodiesel production in tert-amyl alcohol was investigated. The effects of different organic medium, molar ratio of substrate, reaction tempera...
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Article
Preparation of (R)-(−)-mandelic acid and its derivatives from racemates by enantioselective degradation with a newly isolated bacterial strain Alcaligenes sp. ECU0401
An enantioselective mandelate-degrading bacterium, Alcaligenes sp. ECU0401, was newly isolated from soil. By fed-batch culture, (R)-(−)-mandelic acid was successfully prepared in a 5-L fermenter with 32.8% isolat...
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Article
Microbial Ecological Model of Filamentous Bulking and Mechanisms
The paper proposes a prototype mathematical model for the interactions among filamentous bacteria, floc-forming bacteria and protozoa within completely mixed activated sludge systems, which is capable of descr...
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Article
Mathematical Model and Mechanisms for Biofilm Wastewater Treatment Systems
Fundamental theoretical depiction is still lacking on biofilm wastewater treatment systems up to today. A mathematical model of biofilm wastewater treatment systems, taking account of suspended microorganisms ...
