16 Result(s)
within
Leonardo Perez de Souza
-
Article
Anti-Candida sp. activity of Astronium urundeuva derivatives free and loaded into a nanostructured lipid system
Infections caused by Candida species increased, as well as species resistant to the most conventional antimicrobials. Thus, the search for new natural sources of antimicrobials without adverse effects also increa...
-
Article
Open AccessGrowth in fluctuating light buffers plants against photorespiratory perturbations
Photorespiration (PR) is the pathway that detoxifies the product of the oxygenation reaction of Rubisco. It has been hypothesized that in dynamic light environments, PR provides a photoprotective function. To ...
-
Protocol
Databases and Tools to Investigate Protein-Metabolite Interactions
Protein-metabolite interactions (PMIs) are directly responsible for the regulation of numerous processes. From the direct regulation of enzymes to complex developmental processes intermediated by hormones, PMI...
-
Article
Open AccessAzacytidine arrests ripening in cultivated strawberry (Fragaria × ananassa) by repressing key genes and altering hormone contents
Strawberry ripening involves a number of irreversible biochemical reactions that cause sensory changes through accumulation of sugars, acids and other compounds responsible for fruit color and flavor. The proc...
-
Article
Open AccessThe AtMYB60 transcription factor regulates stomatal opening by modulating oxylipin synthesis in guard cells
Stomata are epidermal pores formed by pairs of specialized guard cells, which regulate gas exchanges between the plant and the atmosphere. Modulation of transcription has emerged as an important level of regul...
-
Protocol
Measurement of Flower Metabolite Concentrations Using Gas Chromatography–Mass Spectrometry and High-Performance Liquid Chromatography–Mass Spectrometry
Metabolite profiling aiming at quantifying the metabolome of flowers is emerging as a suitable tool to understand the metabolic complexity of these reproductive organs and the associations between primary and ...
-
Article
Open AccessMulti-omics approaches explain the growth-promoting effect of the apocarotenoid growth regulator zaxinone in rice
The apocarotenoid zaxinone promotes growth and suppresses strigolactone biosynthesis in rice. To shed light on the mechanisms underlying its growth-promoting effect, we employed a combined omics approach integ...
-
Article
Ultra-high-performance liquid chromatography high-resolution mass spectrometry variants for metabolomics research
Ultra-high-performance liquid chromatography high-resolution mass spectrometry (UHPLC–HRMS) variants currently represent the best tools to tackle the challenges of complexity and lack of comprehensive coverage...
-
Article
Mass spectrometry-based metabolomics: a guide for annotation, quantification and best reporting practices
Mass spectrometry-based metabolomics approaches can enable detection and quantification of many thousands of metabolite features simultaneously. However, compound identification and reliable quantification are...
-
Article
Open AccessGlobal map** of protein–metabolite interactions in Saccharomyces cerevisiae reveals that Ser-Leu dipeptide regulates phosphoglycerate kinase activity
Protein–metabolite interactions are of crucial importance for all cellular processes but remain understudied. Here, we applied a biochemical approach named PROMIS, to address the complexity of the protein–smal...
-
Article
Open AccessEvidence of drought memory in Dipteryx alata indicates differential acclimation of plants to savanna conditions
The remarkable phytogeographic characteristics of the Brazilian savanna (Cerrado) resulted in a vegetation domain composed of plants with high structural and functional diversity to tolerate climate extremes. ...
-
Article
Open AccessA cold change: how short low temperature exposure affects primary metabolism in leaves and stems of two eucalyptus species
Plants often modify their metabolism in order to regain homeostasis and maintain survival in the face of stressful conditions. Here, two species of eucalyptus, E. globulus and E. grandis (adapted and non-adapted ...
-
Article
Open AccessOn the natural diversity of phenylacylated-flavonoid and their in planta function under conditions of stress
Plants contain light signaling systems and undergo metabolic perturbation and reprogramming under light stress in order to adapt to environmental changes. Flavonoids are one of the largest classes of natural p...
-
Protocol
Carbon Atomic Survey for Identification of Selected Metabolic Fluxes
Stable-isotope-labeling experiments have become a frequently used tool to investigate different metabolic systems. They have been recently applied to several comprehensive studies in plant metabolomics providi...
-
Protocol
Gas Chromatography–Mass Spectrometry-Based 13C-Labeling Studies in Plant Metabolomics
Stable-isotope labeling analysis has been used to discover new metabolic pathways and their key regulatory points in a wide range of organisms. Given the complexity of the plant metabolic network, this analysi...
-
Protocol
13CO2 Labeling and Mass Spectral Analysis of Photorespiration
Photorespiratory metabolism is compartmented over the chloroplast, peroxisome, cytosol, and mitochondria, and due to its complex structure it is often the case that metabolite levels alone are not able to full...
