17 Result(s)

Protocol

Human-Induced Pluripotent Stem Cell (hiPSC)-Derived Neurons and Glia for the Elucidation of Pathogenic Mechanisms in Alzheimer’s Disease

Alzheimer’s disease (AD) is a common neurodegenerative disorder and a mechanistically complex disease. For the last decade, human models of AD using induced pluripotent stem cells (iPSCs) have emerged as a pow...

Jessica E. Young, Lawrence S. B. Goldstein in Alzheimer’s Disease (2023)

Article

Amyloid-β-independent regulators of tau pathology in Alzheimer disease

The global epidemic of Alzheimer disease (AD) is worsening, and no approved treatment can revert or arrest progression of this disease. AD pathology is characterized by the accumulation of amyloid-β (Aβ) plaqu...

Rik van der Kant, Lawrence S. B. Goldstein, Rik Ossenkoppele in Nature Reviews Neuroscience (2020)

Article

Chromatin establishes an immature version of neuronal protocadherin selection during the naive-to-primed conversion of pluripotent stem cells

In the mammalian genome, the clustered protocadherin (cPCDH) locus provides a paradigm for stochastic gene expression with the potential to generate a unique cPCDH combination in every neuron. Here we report a ch...

Angels Almenar-Queralt, Daria Merkurjev, Hong Sook Kim, Michael Navarro… in Nature Genetics (2019)

Chapter

Human Stem Cell Approaches to Understanding and Treating Alzheimer’s Disease

This chapter describes how human induced pluripotent stem cell (hIPSC) technologies might be used to study Alzheimer’s disease. I argue that significant mechanistic and therapeutic insights may emerge regardin...

Lawrence S. B. Goldstein in Programmed Cells from Basic Neuroscience to Therapy (2013)

Article

Probing sporadic and familial Alzheimer’s disease using induced pluripotent stem cells

Induced pluripotent stem cells are shown to be useful for studying phenotypes relevant to familial and sporadic Alzheimer’s disease, even though it can take decades for the disease to manifest in patients.

Mason A. Israel, Shauna H. Yuan, Cedric Bardy, Sol M. Reyna, Yangling Mu… in Nature (2012)

Article

Somatic coding mutations in human induced pluripotent stem cells

Defined transcription factors can induce epigenetic reprogramming of adult mammalian cells into induced pluripotent stem cells. Although DNA factors are integrated during some reprogramming methods, it is unkn...

Athurva Gore, Zhe Li, Ho-Lim Fung, Jessica E. Young, Suneet Agarwal… in Nature (2011)

Article

Kinesin-mediated axonal transport of a membrane compartment containing β-secretase and presenilin-1 requires APP

Proteolytic processing of amyloid precursor protein (APP) generates amyloid-β peptide and has been implicated in the pathogenesis of Alzheimer's disease¹. However, the normal function of APP, whether this functio...

Adeela Kamal, Angels Almenar-Queralt, James F. LeBlanc, Elizabeth A. Roberts… in Nature (2001)

Article

ADP-induced rocking of the kinesin motor domain revealed by single-molecule fluorescence polarization microscopy

Kinesin is an ATP-driven molecular motor protein that moves processively along microtubules. Despite considerable research, the detailed mechanism of kinesin motion remains elusive. We applied an enhanced suit...

Hernando Sosa, Erwin J.G. Peterman, W.E. Moerner… in Nature Structural Biology (2001)

Article

The muscle in kinesin

The first high resolution structures of the kinesin and NCD motor proteins reveal their surprising similarity to myosin but leave open the tantalizing question of what properties determine the directionality o...

Roman Sakowicz, Lawrence S.B. Goldstein in Nature Structural Biology (1996)

Article

New cytoskeletal liaisons

Lawrence S. B. Goldstein, Ron D. Vale in Nature (1992)

Article

A brave new world for dynein

Lawrence S. B. Goldstein, Ron D. Vale in Nature (1991)

Article

Bead movement by single kinesin molecules studied with optical tweezers

KINESIN, a mechanoenzyme that couples ATP hydrolysis to movement along microtubules, is thought to power vesicle transport and other forms of microtubule-based motility^1–6. Here, microscopic silica beads⁷ were pr...

Steven M. Block, Lawrence S. B. Goldstein, Bruce J. Schnapp in Nature (1990)

Article

Identification of globular mechanochemical heads of kinesin

KINESIN is a mechanoenzyme which uses energy liberated from ATP hydrolysis to transport particles towards the 'plus ends' of microtubules^1–6. The enzyme consists of two polypeptide heavy chains of relative molecu...

Jonathan M. Scholey, John Heuser, Joy T. Yang, Lawrence S. B. Goldstein in Nature (1989)

Article

Mechanisms of chromosome orientation revealed by two meiotic mutants in Drosophila melanogaster

Two disjunction defective meiotic mutants, ord and mei-S332, each of which disrupts meiosis in both male and female Drosophila melanogaster, were analyzed cytologically and genetically in the male germ-line. It w...

Lawrence S. B. Goldstein in Chromosoma (1980)