29 Result(s)

Article

Introduction to the Special Issue on Protein Folding and Harold A. Scherega

Mahesh Narayan, Lawrence J. Berliner in The Protein Journal (2020)

Article

Conformation-Dependent Interaction of α-Lactalbumin with Model and Biological Membranes: A Spin-Label ESR Study

α-Lactalbumin (α-LA) is biosynthesized and stored at the smooth endoplasmic reticulum (ER), then transferred to the Golgi lumen when prolactin stimulation of lactose biosynthesis and secretion takes place. Bec...

Dipankar Chaudhuri, Mahesh Narayan, Lawrence J. Berliner in The Protein Journal (2004)

Article

Thrombin Inhibitor Design: X-Ray and Solution Studies Provide a Novel P1 Determinant

The crystal structures of proflavin and 6-fluorotryptamine thrombin have been completed showing binding of both ligands at the active site S1 pocket. The structure of proflavin:thrombin was confirmatory, while...

Vicki L. Nienaber, Paul D. Boxrud, Lawrence J. Berliner in Journal of Protein Chemistry (2000)

Article

Atomic Structures of Two Nitroxide Spin Labels Complexed with Human Thrombin: Comparison with Solution Studies

Crystal structures of thrombin complexed with two spin labels called para-V, 4-(2,2,5,5-tetramethyl-pyrrolidine-1-oxyl)-p-(fluorosulfonyl) benzamidine, and meta-V, 3-(2,2,5,5-tetramethyl-pyrrolidine-1-oxyl)-m-(fl...

Vicki L. Nienaber, Lawrence J. Berliner in Journal of Protein Chemistry (2000)

Chapter

Riboflavin-Sensitized Singlet Oxygen Formation in Milk

The photosensitized oxidation of foods is initiated by natural pigments or sensitizers producing an excited state which may follow Type I or Type II reaction pathways¹. The interaction of sensitizer with food com...

Lawrence J. Berliner, Tateaki Ogata in Food and Free Radicals (1997)

Article

Preparation and characterization of a biologically active spin-labeled sea anemone toxin

A derivative of the polypeptide cardiostimulant anthopleurin-B(AP-B) labeled with the spin label 1-oxyl 2,2,6,6-tetramethyl-4-piperidinyloxycarbonyl azide has been prepared and characterized. The product was f...

Stephen A. Monks, Raymond S. Norton, Cyril C. Curtain… in Journal of Protein Chemistry (1996)

Article

Comparison of the active-site conformations of bovine α-thrombin and meizothrombin(desF1) by electron spin resonance

The active site of the prothrombin activation intermediate meizothrombin(desF1) was probed using several fluorosulfonylphenyl spin labels specific for the active serine hydroxyl of serine proteases. The mobili...

Paul D. Boxrud, Lawrence J. Berliner in Journal of Protein Chemistry (1996)

Article

Co²⁺ binding to α-lactalbumin

α-Lactalbumin possesses multiple Zn²⁺ binding sites, with the strongest site having an affinity constant of 5×10⁵ M⁻¹ [Permyakovet al. (1991),J. Protein Chem. 100, 577]. The binding of zinc at secondary sites is...

Eugene A. Permyakov, Lawrence J. Berliner in Journal of Protein Chemistry (1994)

Article

Effects of Zn(II) on galactosyltransferase activity

The enzyme β-4-galactosyltransferase (GT) catalyzes the transfer of a galactosyl group from UDP-galactose to N-acetylglucosamine (GlcNAc) on glycoproteins. In the presence of α-lactalbumin (α-LA), galactosyltr...

Eugene A. Permyakov, Irene L. Reyzer, Lawrence J. Berliner in Journal of Protein Chemistry (1993)