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Article
The spectrum of mutations in genes associated with resistance to rifampicin, isoniazid, and fluoroquinolones in the clinical strains of M. tuberculosis reflects the transmissibility of mutant clones
To study the transmissibility of drug resistant mutant clones, M. tuberculosis samples were isolated from the patients of the clinical department and the polyclinic of the Central TB Research Institute (n = 1455)...
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Article
Antibacterial Effects of Liposomes Containing Phospholipid Cardiolipin and Fluoroquinolone Levofloxacin on Mycobacterium tuberculosis with Extensive Drug Resistance
The effects of liposomes containing phospholipid cardiolipin without antibiotic and loaded with levofloxacin on the growth of Mycobacterium tuberculosis with extensive drug resistance were studied in vitro. Lipos...
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Article
New 5-modified 2′-deoxyuridine derivatives: synthesis and antituberculosis activity
New 2′-deoxyuridine derivatives containing long-chain hydrophilic substituents or hydrophilic groups at the 5 position of uracil were synthesized. 5-[4-(2-Carboxyethylcarbonylamino)-butyloxymethyl]-2′-deoxyuri...
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Article
Synthesis and biological properties of α-thymidine 5′-aryl phosphonates
Diethyl(N-arylaminocarbonyl)methyl phosphonates have been obtained by the reaction of diethylphosphonoacetic acid imidazolides with methyl-4-aminobenzoate or 3,5-bis(trifluoromethyl)phenylamine. Their treatment w...
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Article
Performance of Cepheid® Xpert MTB/RIF® and TB-Biochip® MDR in two regions of Russia with a high prevalence of drug-resistant tuberculosis
The purpose of this study was to assess the performance of Cepheid® Xpert MTB/RIF® (“Xpert”) and TB-Biochip® MDR (“TB-Biochip”). Sputum specimens from adults with presumptive tuberculosis (TB) were homogenized...
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Article
Molecular ty** of Mycobacterium tuberculosis circulated in Moscow, Russian Federation
The present study investigates epidemiological diversity and multidrug resistance spreading among Mycobacterium tuberculosis strains circulating in Moscow, Russian Federation. Among 115 M. tuberculosis strains se...
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Article
Effect of exogenous cardiolipin on the growth and viability of Mycobacterium tuberculosis H37Rv in vitro
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Article
Effects of Liposomes of Different Lipid Composition on In Vitro Growth of Mycobacterium Tuberculosis H37Rv
The growth of M. tuberculosis H37RV in culture medium was studied after addition of liposomes from different lipids (phosphatidylcholine, cardiolipin, and glycosphyngolipids). Addition of phosphatidylcholine into...
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Article
Detection of mutations in Mycobacterium tuberculosis genome determining resistance to fluoroquinolones by hybridization on biological microchips
We developed a method of identification of Mycobacterium tuberculosis with simultaneous evaluation of the sensitivity to fluoroquinolones on a biological microchip array. The method of multiplex two-staged PCR fo...
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Article
Ex vivo production of interferon-γ, tumor necrosis factor-α, and interleukin-6 by mouse macrophages during infection with M. bovis and M. tuberculosis H37Rv
Ex vivo production of IFN-γ, TNF-α, and IL-6 by mouse peritoneal macrophages was studied during successive infection with the vaccine strain M. bovis BCG and virulent strain M. tuberculosis H37Rv...
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Article
Detection of Rifampicin-Resistant Mycobacterium tuberculosis Strains by Hybridization and Polymerase Chain Reaction on a Specialized TB-Microchip
Two alternative methods for identification of rifampicin-resistant strains of Mycobacterium tuberculosis on biological microchips are developed. The methods are based on detection of point mutations and other ...
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Article
Use of allele-specific amplification and analysis of conformation polymorphism for detecting rifampicin resistance of clinical strains ofMycobacterium tuberculosis
Analysis of 90 clinical strains ofM. tuberculosis demonstrated the possibility of using allele-specific amplification for detecting mutations determining rifampicin resistance. Successive use of two allele-specif...
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Article
Epitopic map** of different mycobacteria with the use of monoclonal antibodies
Monoclonal antibodies reacting with different mycobacteria are prepared. They are used for epitopic map** ofMycobacteria tuberculosis H37Rv, M. bovis bovinus 8, M. avium, M. kansasii, andM. smegmatis. On the ba...
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Article
Preparation and characterization of murine monoclonal antibodies toSalmonella typhimurium K-antigen
Monoclonal antibodies toS. typhimurium K-antigens representing IgG1 immunoglobulins with a “kappa”-type light chain are prepared and characterized. They are highly active in indirect enzyme immunoassay with purif...
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Article
Effect of antiserum against isologous aggregated immunoglobulins on synthesis of nonspecific immunoglobulins in mice immunized with Sheep's red blood cells
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Article
Effect of antiserum against isologous aggregated immunoglobulins on delayed-type hypersensitivity reaction in mice immunized with sheep red blood cells
Injection of mouse antiserum against isologous aggregated immunoglobulins (MAAS) into mice previously receiving 105 sheep red blood cells (SRBC) did not affect the intensity of the delayed-type hypersensitivity (...
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Article
Effect of mouse antiserum against isologous aggregated immunoglobulins on accumulation of rosette-forming and antibody-forming cells in mice immunized with sheep's red blood cells
Data are given on the effect of mouse antiserum against isologous aggregated immunoglobulins (MAAS) on the kinetics of rosette-forming (RFC) and antibody-forming cells (AFC) in mice immunized with sheep's red ...
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Article
Preparation of a mouse antiserum against isologous aggregated immunoglobulins and the study of its action on rosette-forming cells
A method of obtaining antiserum against isologous aggregated mouse immunoglobulins (MAAS) is described. This serum blocks the antigen-binding receptors of immune rosette-forming cells in vitro. MAAS was inject...
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Article
Antigen-dependent induction of a nonspecific humoral factor blocking rosette-forming cells in vitro and in vivo
Isologous serum of mice immunized with rabbit globulin (IARS) was shown to contain a factor inactivating rosette-forming cells (RFC) in vitro from CBA mice immunized with sheep's erythrocytes. If the mice were...
