-
Article
Effect of DNA methylation on the interaction with tetrahydrocortisol–apolipoprotein A-I complex
The effect of GCNGC site methylation on the DNA binding to tetrahydrocortisol–apolipoprotein A-I complex was shown by atomic force microscopy and enzymatic analysis. Methylation of GCNGC sites alters the mecha...
-
Article
The action of androgens on Na+,K+-ATPase activity of erythrocyte membranes
The effect of androgens (testosterone, androsterone, dehydroepiandrosterone and dehydroepiandrosterone sulfate) on erythrocyte membrane during their nonspecific binding was investigated. The change in erythroc...
-
Article
Apolipoprotein A-I as a Carrier of Lipopolysaccharide into Rat Hepatocytes
Apolipoprotein A-I-mediated transport of LPS into isolated rat hepatocytes was demonstrated by means of fluorescent microscopy and spectrofl uorometry. The efficiency of intracellular endotoxin transport in a ...
-
Article
Activity of Matrix Metalloproteinases during Antimycobacterial Therapy in Mice with Simulated Tuberculous Inflammation
Matrix metalloproteinases are shown to be involved in the pathogenesis of tuberculosis inflammation. In the early stages of BCG-granuloma formation in mouse liver and lungs, the serum levels of matrix metallop...
-
Article
Plasma Lipoproteins as a Transport Form of Extracellular DNA
We showed DNA-binding activity of different classes of plasma lipoproteins in rats and humans. Experiments with fluorescent dye Hoechst 33258 showed that about 12% extracellular plasma DNA is present in circul...
-
Article
Analysis of Serum Activities of Matrix Metalloproteinases and α1-Proteinase Inhibitor in Patients with Type 2 Diabetes Mellitus
Activities of matrix metalloproteinases 2 and 7 and α1-proteinase inhibitor were measured in patients with type 2 diabetes mellitus. Correlations between enzyme activity and the main parameters of carbohydrate...
-
Article
Interaction of corundum and quartz nanocrystals with erythrocyte membranes
Mechanisms of interaction of quartz and corundum nanocrystals with erythrocyte membranes were studied by means of atomic force microscopy and fluorescence analysis. Hydrophobic, chemically inert nanocrystals l...
-
Article
The analysis of interaction of lipoproteins and steroid hormones
Using the methods of gel-filtration and fluorescence quenching, it has been demonstrated that plasma lipoproteins bind steroid hormones and can therefore play a role in their active transport in the body. High...
-
Article
Effect of Blood Lipoproteins and Apolipoproteins A-I, C, and E on the Microviscosity of Erythrocyte Membranes
We studied in vitro modifying effect of high-density lipoproteins, low-density lipoproteins, very-low-density lipoproteins, and apolipoproteins A-I, C, and E on membrane structure of rat erythrocytes. Incubation ...
-
Article
Effect of Apolipoprotein A-I Complex with Tetrahydrocortisone on Protein Biosynthesis and Glucose Absorption by Rat Hepatocytes
We studied the effect of apolipoprotein A-I—tetrahydrocortisone complex on 14C glucose absorption and lactate accumulation and on the rate of protein biosynthesis in isolated rat hepatocytes. The presence of apol...
-
Article
Effect of Plasma Lipoproteins and Their Complexes with Polysaccharides on Interleukin-1β Concentration in Macrophages of Mice with HA-1 Ascitic Hepatoma
Experiments on cultured peritoneal macrophage from mice with HA-1 ascitic hepatoma showed that plasma lipoproteins present in the incubation medium decreased intracellular concentration of interleukin-1β. Thes...
-
Article
Mechanisms determining phenotypic heterogeneity of hepatocytes
This review summarizes results of biochemical and immunohistochemical studies indicating the existence of functional heterogeneity of hepatocytes depending on their localization in the hepatic acinus; this det...
-
Article
Role of glucocorticoids and resident liver macrophages in induction of tyrosine aminotransferase
Administration of cortisol to an animal induces tyrosine aminotransferase (TAT) in the liver. A similar effect was observed after stimulation of resident liver macrophages (Kupffer cells) by dextran sulfate. A...
-
Article
Mechanisms of interaction of tetrahydrocortisol and its ApoA-I complex with a model DNA regulatory element CC(GCC)5/GG(CGG)5
Interaction of tetrahydrocortisol (THF) and its apolipoprotein A-I (ApoA-I) complex with a CC(GCC)5/GG(CGG)5 duplex were studied by IR spectroscopy, which revealed formation of hydrogen bonds between the OH group...
-
Article
Interaction of apolipoproteins A-I and E in the regulation of DNA, RNA, and protein biosynthesis in cultured rat hepatocytes
Experiments on hepatocyte culture showed that apolipoprotein A-I—tetrahydrocortisol complex increases the rate of DNA, RNA, and protein biosynthesis measured by radioactive label incorporation. Apolipoprotein ...
-
Article
Effect of complexes of apolipoprotein A-I with tetrahydrocortisol and pregnenolone on protein biosynthesis in rat hepatocytes culture
Complexes of apolipoprotein A-I with tetrahydrocortisol and pregnenolone exhibit high biological activity and increase the rate of protein biosynthesis in the culture of rat hepatocytes. An important role in t...
-
Article
Structure of eukaryotic DNA binding sites for steroid hormone-apolipoprotein A-I complexes
High affinity for DNA and synthetic oligonucleotides was detected for apolipoprotein A-I (ApoA-I) by affinity chromatography, affinity modification, and enzymatic analysis. Competitive inhibition and Southern ...
-
Article
Characteristics of the interactions of cortisol-apolipoprotein A-I and tetrahydrocortisol-apolipoprotein A-I complexes with eukaryotic DNA
Primary hepatocyte culture has been used to demonstrate that the cortisol-apolipoprotein A-I complex does not affect the DNA and protein biosynthesis rates, whereas the tetrahydrocortisol-apolipoprotein A-I co...
-
Article
Apolipoprotein A-I Possessing Activity of Defensin Proteins Modifies Structure of Plasma Membranes
We studied the in vitro effect of apolipoprotein A-I possessing activity of defensin proteins on the structure of plasma membranes in donor erythrocytes and lymphocytes. Incubation of erythrocyte membranes with a...
-
Article
Effect of Polysaccharides and Human Plasma Lipoproteins on the Secretion of Cystatin C by Peritoneal Macrophages from Normal and Tumor Bearing Mice
Intact peritoneal macrophages in vitro secreted the cysteine proteinase inhibitor cystatin C. Polysaccharides stimulated cystatin C secretion: lipopolysaccharide < carboxymethylated β-D-glucan < sulfoethylated β-...
