5 Result(s)
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Article
Open AccessANCA: artificial nucleic acid circuit with argonaute protein for one-step isothermal detection of antibiotic-resistant bacteria
Endonucleases have recently widely used in molecular diagnostics. Here, we report a strategy to exploit the properties of Argonaute (Ago) proteins for molecular diagnostics by introducing an artificial nucleic...
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Article
Open AccessDesign of hypoxia responsive CRISPR-Cas9 for target gene regulation
The CRISPR–Cas9 system is a widely used gene-editing tool, offering unprecedented opportunities for treating various diseases. Controlling Cas9/dCas9 activity at specific location and time to avoid undesirable...
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Article
Open AccessTetrameric architecture of an active phenol-bound form of the AAA+ transcriptional regulator DmpR
The Pseudomonas putida phenol-responsive regulator DmpR is a bacterial enhancer binding protein (bEBP) from the AAA+ ATPase family. Even though it was discovered more than two decades ago and has been widely used...
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Article
Open AccessIn vivo genome editing using the Cpf1 ortholog derived from Eubacterium eligens
Cpf1 is an RNA-guided endonuclease that can be programmed to cleave DNA targets. Specific features, such as containing a short crRNA, creating a staggered cleavage pattern and having a low off-target rate, ren...
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Article
Open AccessStepwise heating in Stille polycondensation toward no batch-to-batch variations in polymer solar cell performance
For a given π-conjugated polymer, the batch-to-batch variations in molecular weight (Mw) and polydispersity index (Ð) can lead to inconsistent process-dependent material properties and consequent performance vari...
