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Article
Highly efficient and specific regulation of gene expression using enhanced CRISPR-Cas12f system
The recently developed CRISPR activator (CRISPRa) system uses a CRISPR-Cas effector-based transcriptional activator to effectively control the expression of target genes without causing DNA damage. However, ex...
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Article
Open AccessEnhancing the thermostability and activity of glycosyltransferase UGT76G1 via computational design
The diterpene glycosyltransferase UGT76G1, derived from Stevia rebaudiana, plays a pivotal role in the biosynthesis of rebaudioside A, a natural sugar substitute. Nevertheless, its potential for industrial applic...
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Article
Open AccessANCA: artificial nucleic acid circuit with argonaute protein for one-step isothermal detection of antibiotic-resistant bacteria
Endonucleases have recently widely used in molecular diagnostics. Here, we report a strategy to exploit the properties of Argonaute (Ago) proteins for molecular diagnostics by introducing an artificial nucleic...
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Article
Open AccessDesign of hypoxia responsive CRISPR-Cas9 for target gene regulation
The CRISPR–Cas9 system is a widely used gene-editing tool, offering unprecedented opportunities for treating various diseases. Controlling Cas9/dCas9 activity at specific location and time to avoid undesirable...
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Article
Author Correction: Hypercompact adenine base editors based on a Cas12f variant guided by engineered RNA
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Article
Hypercompact adenine base editors based on a Cas12f variant guided by engineered RNA
Cas12f is a hypercompact type V, Cas12 family member. Previously, we reported a set of engineered guide RNAs supporting high indel efficiency for Cas12f1 in human cells. Here we suggest a new technology whereb...
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Article
Directed evolution of glycosyltransferase for enhanced efficiency of avermectin glucosylation
Avermectin, produced by Streptomyces avermitilis, is an active compound protective against nematodes, insects, and mites. However, its potential usage is limited by its low aqueous solubility. The uridine diphosp...
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Article
Open AccessTetrameric architecture of an active phenol-bound form of the AAA+ transcriptional regulator DmpR
The Pseudomonas putida phenol-responsive regulator DmpR is a bacterial enhancer binding protein (bEBP) from the AAA+ ATPase family. Even though it was discovered more than two decades ago and has been widely used...
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Article
Oral administration of Ulmus davidiana extract suppresses interleukin-1β expression in LPS-induced immune responses and lung injury
Ulmus davidiana (UD) is a traditional Korean herb medicine that is used to treat inflammatory disorders. UD has been shown to modulate a number of inflammatory processes in vitro or in vivo studies. However, the ...
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Article
Open AccessIn vivo genome editing using the Cpf1 ortholog derived from Eubacterium eligens
Cpf1 is an RNA-guided endonuclease that can be programmed to cleave DNA targets. Specific features, such as containing a short crRNA, creating a staggered cleavage pattern and having a low off-target rate, ren...
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Article
Open AccessStepwise heating in Stille polycondensation toward no batch-to-batch variations in polymer solar cell performance
For a given π-conjugated polymer, the batch-to-batch variations in molecular weight (Mw) and polydispersity index (Ð) can lead to inconsistent process-dependent material properties and consequent performance vari...
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Article
A novel geometric invariant of a pair of conics and its application to eyeglasses-tracking interface
This paper deals with a three-dimensional (3-D) pose estimation problem of a pair of two conics. We first introduce a novel geometric invariant derived from a pair of conics, and then propose a procedure of de...
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Chapter and Conference Paper
Korean Word Search Interface for Wearable Computers Using a Wrist-Mounted Camera Device
This paper presents a hand shape recognition method and its application to Korean word search interface for wearable computers. We first describe a hand shape recognition method composed of hand region segment...
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Article
Immersive teleconference system based on human-robot-avatar interaction using head-tracking devices
This paper introduces a cost-efficient immersive teleconference system. Especially to enhance immersive interaction capability during one-to-many telecommunication, this paper concentrates on the design of a t...
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Article
Methyl β-cyclodextrin activates eNOS-cGMP pathway in endothelial cells
Cyclodextrins (CDs) have utility for solubilizing and stabilizing drugs. Recently CDs have been foused as therapeutics for neurodegenerative diseases. However, some CDs are toxic when administered parenterally...
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Article
Inhibitory effect of dibutyryl chitin ester on nitric oxide and prostaglandin E2 production in LPS-stimulated RAW 264.7 cells
Inflammation is a highly complex process that protects against foreign challenge or tissue injury. The ester derivative dibutyryl chitin (DBC) reportedly accelerates wound healing and exerts an anti-inflammato...
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Article
Effective microorganism substance (EM-S) reduces development and aggravation of atopic dermatitis-like skin lesions in NC/Nga mice
In a previous study, our group showed that the effective microorganism substance (EM-S) produced by fermentation of medicinal plants with effective microorganisms has an antiinflammatory effect on atopic derma...
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Chapter and Conference Paper
Gesture-Based Interface Using Baby Signs for the Elderly and People with Mobility Impairment in a Smart House Environment
We present a development of a gesture-based interface for the elderly and people with mobility impairment in a smart house environment. To reduce a burden of memorizing a variety of gesture commands, we introd...
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Article
Beat gesture recognition and finger motion control of a piano playing robot for affective interaction of the elderly
This paper introduces a piano playing robot in views of smart house and assistive robot technology to care the affective states of the elderly. We address the current issues in this research area and propose a...
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Article
Open AccessIntelligent Interaction for Human-Friendly Service Robot in Smart House Environment
The smart house under consideration is a service-integrated complex system to assist older persons and/or people with disabilities. The primary goal of the system is to achieve independent living by various ro...
