3 Result(s)
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Article
Open AccessCooperative effects of oocytes and estrogen on the forkhead box L2 expression in mural granulosa cells in mice
Forkhead box L2 (FOXL2) plays a critical role in the development and function of mammalian ovaries. In fact, the causative effects of FOXL2 misregulations have been identified in many ovarian diseases, such as...
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Article
Open AccessGeneration of genetically modified mice using SpCas9-NG engineered nuclease
Although genetically modified mice can be generated with high efficiency by using CRISPR/Cas9-mediated genome editing in mouse zygotes, only the loci with a protospacer-adjacent motif (PAM) sequence are target...
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Article
Open AccessDevelopment of a mono-promoter-driven CRISPR/Cas9 system in mammalian cells
The CRISPR/Cas9 system has been used for spatio-temporal gene modification through the ubiquitous expression of gRNA by an RNA polymerase III promoter and the controlled expression of Cas9 using a tissue-speci...
