Plant Gene Silencing
Methods and Protocols
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Kirankumar S. Mysore
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Article
Open AccessCorrection: Comprehensive genomic analysis of Bacillus subtilis and Bacillus paralicheniformis associated with the pearl millet panicle reveals their antimicrobial potential against important plant pathogens
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Article
Open AccessComprehensive genomic analysis of Bacillus subtilis and Bacillus paralicheniformis associated with the pearl millet panicle reveals their antimicrobial potential against important plant pathogens
Plant microbiome confers versatile functional roles to enhance survival fitness as well as productivity. In the present study two pearl millet panicle microbiome member species Bacillus subtilis PBs 12 and Bacill...
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Open AccessThe long noncoding RNA LAL contributes to salinity tolerance by modulating LHCB1s’ expression in Medicago truncatula
Long non-coding RNAs (lncRNAs) are abundant in plants, however, their regulatory roles remain unclear in most biological processes, such as response in salinity stress which is harm to plant production. Here w...
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Open AccessAgrobacterium expressing a type III secretion system delivers Pseudomonas effectors into plant cells to enhance transformation
Agrobacterium-mediated plant transformation (AMT) is the basis of modern-day plant biotechnology. One major drawback of this technology is the recalcitrance of many plant species/varieties to Agrobacterium infect...
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Protocol
Virus-Induced Gene Silencing in Sorghum Using Brome Mosaic Virus
Sorghum [Sorghum bicolor (L.) Moench.] is a versatile crop, grown in 30 countries and a food source for nearly 500 million people globally. Although the sorghum genome is sequenced, a limited understanding of gen...
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Protocol
High-Throughput Analysis of Gene Function under Multiple Abiotic Stresses Using Leaf Disks from Silenced Plants
The high throughputness and affordability of “omics” technologies is leading to the identification of a large number of abiotic stress genes, with many of them responsive to multiple stresses. In vivo function...
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Chapter
Whole Genome Sequencing Identifies a Medicago truncatula Tnt1 Insertion Mutant in the VTL8 Gene that is Essential for Symbiotic Nitrogen Fixation
Legumes carry out symbiotic nitrogen (N) fixation by forming N-fixing nodules with rhizobia during N deficiency. In an effort to discover new legume genes essential to nodule function using the Medicago truncatul...
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Chapter
Tnt1 Insertional Mutagenesis in Medicago truncatula for Gene Function Analysis
Legumes play a key role in the sustainable agriculture to fix atmospheric nitrogen through a mutualistic symbiotic process with nitrogen-fixing bacteria. Medicago truncatula is a diploid model legume and has high...
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Protocol
Recent Advances in Plant Gene Silencing Methods
With the increasing understanding of fundamentals of gene silencing pathways in plants, various tools and techniques for downregulating the expression of a target gene have been developed across multiple plant...
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Book
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Article
The 3-ketoacyl-CoA synthase WFL is involved in lateral organ development and cuticular wax synthesis in Medicago truncatula
A 3-ketoacyl-CoA synthase involved in biosynthesis of very long chain fatty acids and cuticular wax plays a vital role in aerial organ development in M. truncatula.
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Open AccessMedicago PHYA promotes flowering, primary stem elongation and expression of flowering time genes in long days
Flowering time is an important trait for productivity in legumes, which include many food and fodder plants. Medicago truncatula (Medicago) is a model temperate legume used to study flowering time pathways. Like
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A molecular framework underlying the compound leaf pattern of Medicago truncatula
Compound leaves show more complex patterns than simple leaves, and this is mainly because of a specific morphogenetic process (leaflet initiation and arrangement) that occurs during their development. How the ...
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Article
Open AccessOverexpression of VIRE2-INTERACTING PROTEIN2 in Arabidopsis regulates genes involved in Agrobacterium-mediated plant transformation and abiotic stresses
Arabidopsis VIRE2-INTERACTING PROTEIN2 (VIP2) was previously described as a protein with a NOT domain, and Arabidopsis vip2 mutants are recalcitrant to Agrobacterium-mediated root transformation. Here we show tha...
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Article
Author Correction: NIN interacts with NLPs to mediate nitrate inhibition of nodulation in Medicago truncatula
In the version of this Article originally published, the name of author Zhenpeng Luo was incorrectly listed as Zhenpeng Luo Luo. Additionally, nitrate in Fig. 6b was incorrectly shown as ‘NO3+’ rather than ‘NO3–’...
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NIN interacts with NLPs to mediate nitrate inhibition of nodulation in Medicago truncatula
Legume plants can assimilate inorganic nitrogen and have access to fixed nitrogen through symbiotic interaction with diazotrophic bacteria called rhizobia. Symbiotic nitrogen fixation is an energy-consuming pr...
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Open AccessMiR393 and miR390 synergistically regulate lateral root growth in rice under different conditions
Plants have evolved excellent ability of flexibly regulating the growth of organs to adapt to changing environment, for example, the modulation of lateral root development in response to environmental stresses...
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Open AccessAn efficient and improved method for virus-induced gene silencing in sorghum
Although the draft genome of sorghum is available, the understanding of gene function is limited due to the lack of extensive mutant resources. Virus-induced gene silencing (VIGS) is an alternative to mutant r...
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Protocol
Tnt1 Insertional Mutagenesis in Medicago truncatula
Legumes play irreplaceable roles in sustainable agriculture due to their unique capability of fixing gaseous nitrogen in the atmosphere and turning into plant-usable ammonium through interaction with rhizobia....
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Protocol
Tissue Culture (Somatic Embryogenesis)-Induced Tnt1 Retrotransposon-Based Mutagenesis in Brachypodium distachyon
Brachypodium distachyon is a model grass species for economically important cereal crops. Efforts are in progress to develop useful functional genomic resources in Brachypodium. A tobacco retrotransposon, Tnt1, ...
