-
Article
Open AccessData-driven regularization lowers the size barrier of cryo-EM structure determination
Macromolecular structure determination by electron cryo-microscopy (cryo-EM) is limited by the alignment of noisy images of individual particles. Because smaller particles have weaker signals, alignment errors...
-
Article
Open AccessAutomated model building and protein identification in cryo-EM maps
Interpreting electron cryo-microscopy (cryo-EM) maps with atomic models requires high levels of expertise and labour-intensive manual intervention in three-dimensional computer graphics programs1,2. Here we prese...
-
Article
Open AccessRapid discovery of high-affinity antibodies via massively parallel sequencing, ribosome display and affinity screening
Develo** therapeutic antibodies is laborious and costly. Here we report a method for antibody discovery that leverages the Illumina HiSeq platform to, within 3 days, screen in the order of 108 antibody–antigen ...
