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    Protocol

    DNA Extraction from Natural Environments

    Until recently, studies on microbial communities present in natural environments relied on conventional optical microscopic observation and cultivation-based approaches. Although these traditional approaches r...

    Kenneth D. Bruce, Peter Strike, Donald A. Ritchie in Environmental Monitoring of Bacteria (1999)

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    Protocol

    Fluorescent Polymerase Chain Reaction/Restriction Fragment Length Polymorphism Monitoring of Genes Amplified Directly from Bacterial Communities in Soils and Sediments

    There has been a growing acknowledgment of both the ecological and biotechnological importance of microbes in natural environments. Concerns about the nonrepresentative nature of traditional analytical methods...

    Kenneth D. Bruce, Mark R. Hughes in Environmental Monitoring of Bacteria (1999)