-
Article
Characterization of an immunomodulatory Der p 2-FIP-fve fusion protein produced in transformed rice suspension cell culture
Der p 2, a major allergen of Dermatophagoides pteronyssinus mites, is one of the most clinically relevant allergens to allergic patients worldwide. FIP-fve protein (Fve) from the golden needle mushroom (Flammulin...
-
Article
Open AccessEvaluation of stool microbiota signatures in two cohorts of Asian (Singapore and Indonesia) newborns at risk of atopy
Studies have suggested that demographic and lifestyle factors could shape the composition of fecal microbiota in early life. This study evaluated infant stool microbiota signatures in two Asian populations, Si...
-
Article
Epitope map** and structural analysis of the anti-Der p 1 monoclonal antibody: insight into therapeutic potential
Group 1 allergen from Dermatophagoid pteronyssinus (Der p 1) belongs to the papain-like cysteine protease family and is a major cause of allergic rhinitis and asthma. An anti-Der p 1 monoclonal antibody, mAb W108...
-
Protocol
Production of Monoclonal Antibody by DNA Immunization with Electroporation
DNA immunization with in vivo electroporation is an efficient alternative protocol for the production of monoclonal antibodies (mAb). Generation of mAb by DNA immunization is a novel approach to circumvent the...
-
Article
Effect of a Milk Formula Containing Probiotics on the Fecal Microbiota of Asian Infants at Risk of Atopic Diseases
The fecal microbiota of 37 infants with (n = 20) or without (n = 17) probiotic administration was evaluated on D 3, and at 1, 3, and 12 mo by fluorescence in situ hybridization-flow cytometry (FISH-FC), PCR, and ...
-
Article
Complete 1H, 13C and 15N resonance assignments of Blo t 5, a major mite allergen from Blomia tropicalis
-
Article
Production and purification of recombinant Blomia tropicalis group 5 allergen from Pichia pastoris culture
The gene of Blomia tropicalis group 5 allergen (Blo t 5) was cloned and expressed in Pichia pastoris KM71. Selected KM71 clones were cultivated in a fed-batch bioreactor feeding first glycerol then followed by me...
-
Article
Immunoprophylaxis of allergen–induced immunoglobulin E synthesis and airway hyperresponsiveness in vivo by genetic immunization
The efficacy of an “allergen–gene immunization protocol” in altering allergic response was examined. Intramuscular injection of rats with a plasmid DNA encoding a house dust mite allergen into the muscle resul...
-
Chapter
Conserved Nodulation Genes are Obligatory for Nonlegume Nodulation
The nodulation of the nonlegume plant Parasponia by bacterial strains occurs through a mechanism quite distinct from that used by these same strains to nodulate legume species (1,2,3). In contrast to the invasion...
-
Article
Molecular cloning of a nodulation gene from fast- and slow-growing strains of Lotus rhizobia
Cosmids containing a nodulation gene from Rhizobium loti NZP2037 were isolated using a 12.8 kb nod:: Tn5 EcoRI fragment from the Nod- mutant strain PN233, as a hybridisation probe. A physical map of the nod regio...
