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Article
Open AccessTowards engineering a hybrid carboxysome
Carboxysomes are bacterial microcompartments, whose structural features enable the encapsulated Rubisco holoenzyme to operate in a high-CO2 environment. Consequently, Rubiscos housed within these compartments pos...
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Article
DABs accumulate bicarbonate
A newly discovered energy-linked carbonic anhydrase, DabBA2, is a two-protein complex responsible for inorganic carbon accumulation in the sulfur bacterium Halothiobacillus neapolitanus. DABs are present in a wid...
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Article
Open AccessCarboxysome encapsulation of the CO2-fixing enzyme Rubisco in tobacco chloroplasts
A long-term strategy to enhance global crop photosynthesis and yield involves the introduction of cyanobacterial CO2-concentrating mechanisms (CCMs) into plant chloroplasts. Cyanobacterial CCMs enable relatively ...
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Article
Towards turbocharged photosynthesis
The development of tobacco plants that are genetically engineered to produce a more efficient form of Rubisco, an enzyme involved in photosynthesis, marks a step towards increasing crop yields. See Letter ...
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Article
Identification and characterization of a carboxysomal γ-carbonic anhydrase from the cyanobacterium Nostoc sp. PCC 7120
Carboxysomes are proteinaceous microcompartments that encapsulate carbonic anhydrase (CA) and ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco); carboxysomes, therefore, catalyze reversible HCO3 ...
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Chapter and Conference Paper
The Effects of Antisense Suppression of δ Subunit of Chloroplast ATP Synthase on the Rates of Chloroplast Electron Transport and CO2 Assimilation in Transgenic Tobacco
In C3 plants, CO2 assimilation is limited by RuBP regeneration rate at high CO2. RuBP regeneration rate in turn is determined by either the chloroplast electron transport capacity to generate NADPH and ATP or the...
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Article
Over-expression of the β-carboxysomal CcmM protein in Synechococcus PCC7942 reveals a tight co-regulation of carboxysomal carbonic anhydrase (CcaA) and M58 content
Carboxysomes, containing the cell’s complement of RuBisCO surrounded by a specialized protein shell, are a central component of the cyanobacterial CO2-concentrating mechanism. The ratio of two forms of the β-carb...
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Article
The CO2-concentrating mechanism of Synechococcus WH5701 is composed of native and horizontally-acquired components
The cyanobacterial CO2-concentrating mechanism (CCM) is an effective adaptation that increases the carbon dioxide (CO2) concentration around the primary photosynthetic enzyme Ribulose-1,5-bisphosphate Carboxylase...
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Article
Inorganic carbon transporters of the cyanobacterial CO2 concentrating mechanism
Cyanobacteria possess an environmental adaptation known as a CO2 concentrating mechanism (CCM) that evolved to improve photosynthetic performance, particularly under CO2-limiting conditions. The CCM functions to ...
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Article
Characterisation of CO2 and HCO3 − uptake in the cyanobacterium Synechocystis sp. PCC6803
The availability of a complete genome database for the cyanobacterium Synechocystissp. PCC6803 (glucose-tolerant strain) has raised expectations that this organism would become a reference strain for work aimed a...
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Article
Cloning and expression of a prokaryotic sucrose-phosphate synthase gene from the cyanobacterium Synechocystis sp. PCC 6803
Sucrose is one of several low-molecular-weight compounds that cyanobacteria accumulate in response to osmotic stress and which are believed to act as osmoprotectants. The genome of the cyanobacterium Synechocy...
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Chapter
Involvement of the cmpABCD Genes in Bicarbonate Transport of the Cyanobacterium Synechococcus sp. Strain PCC 7942
Cyanobacteria possess a CO2-concentrating mechanism (CCM) which elevates the CO2 concentration around the active site of Rubisco and thereby compensates for the low selectivity of Rubisco for CO2 [1]. Active tran...
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Chapter
Regulation of CO2 Assimilation Rate by the Chloroplast Cytochrome BF Complex
The photosynthetic electron transport pathway is responsible for the provision of ATP and NADPH for photosynthetic carbon reduction (PCR) and as such it has the potential to exert strong regulation over photos...
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Article
Reduced levels of cytochrome bf complex in transgenic tobacco leads to marked photochemical reduction of the plastoquinone pool, without significant change in acclimation to irradiance
Tobacco transgenics with decreased amounts of the FeS apoprotein were generated using an antisense RNA construct targeted against the nuclear-encoded Rieske FeS protein of cytochrome bf complex [Price et al. (...
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Article
PsaE- and NdhF-mediated electron transport affect bicarbonate transport rather than carbon dioxide uptake in the cyanobacteriumSynechococcus sp. PCC7002
The wild-type and three deletional interposon mutants (psaE, ndhF, psaE/ndhF) ofSynechococcus sp. PCC7002 were analysed with respect to their ability to induce the CO2-concentrating mechanism (CCM). The response ...
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Article
Reduced levels of cytochrome b 6/f in transgenic tobacco increases the excitation pressure on Photosystem II without increasing sensitivity to photoinhibition in vivo
We have examined tobacco transformed with an antisense construct against the Rieske-FeS subunit of the cytochromeb 6 f complex, containing only 15 to 20% of the wild-type level of cytochrome f. The anti-Rieske-Fe...
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Article
Characterisation of carbon dioxide and bicarbonate transport during steady-state photosynthesis in the marine cyanobacterium Synechococcus strain PCC7002
Net O2 evolution, gross CO2 uptake and net HCO inf3 su− uptake during steady-state photosynthesis were investigated by a recently developed mass-spectrometric ...
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Article
Specific reduction of chloroplast glyceraldehyde-3-phosphate dehydrogenase activity by antisense RNA reduces CO2 assimilation via a reduction in ribulose bisphosphate regeneration in transgenic tobacco plants
The reduction of 3-phosphoglycerate (PGA) to triose phosphate is a key step in photosynthesis linking the photochemical events of the thylakoid membranes with the carbon metabolism of the photosynthetic carbon...
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Article
Specific reduction of chloroplast carbonic anhydrase activity by antisense RNA in transgenic tobacco plants has a minor effect on photosynthetic CO2 assimilation
As an approach to understanding the physiological role of chloroplast carbonic anhydrase (CA), this study reports on the production and preliminary physiological characterisation of transgenic tobacco (Nicotiana ...
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Chapter
Conserved Nodulation Genes are Obligatory for Nonlegume Nodulation
The nodulation of the nonlegume plant Parasponia by bacterial strains occurs through a mechanism quite distinct from that used by these same strains to nodulate legume species (1,2,3). In contrast to the invasion...
