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  1. Article

    Open Access

    Synthesis of cyclophosphamide metabolites by a peroxygenase from Marasmius rotula for toxicological studies on human cancer cells

    Cyclophosphamide (CPA) represents a widely used anti-cancer prodrug that is converted by liver cytochrome P450 (CYP) enzymes into the primary metabolite 4-hydroxycyclophosphamide (4-OH-CPA), followed by non-en...

    Susanne Steinbrecht, Jan Kiebist, Rosalie König, Markus Thiessen… in AMB Express (2020)

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  2. No Access

    Chapter

    Fungal Peroxygenases: A Phylogenetically Old Superfamily of Heme Enzymes with Promiscuity for Oxygen Transfer Reactions

    Fungal unspecific peroxygenases (UPOs, EC 1.11.2.1) form a superfamily of heme proteins and possess promiscuity for oxygen transfer reactions. The first UPO was discovered in the agaric fungus Agrocybe aegerita i...

    Martin Hofrichter, Harald Kellner… in Grand Challenges in Fungal Biotechnology (2020)

  3. No Access

    Article

    Back to “once a caesarean: always a caesarean”? A trend analysis in Switzerland

    Caesarean sections (CS) have significantly increased worldwide and a previous CS is nowadays an important and increasingly reported indication to perform a repeat CS. There is a paucity of information in Switz...

    Corina Christmann-Schmid, Luigi Raio… in Archives of Gynecology and Obstetrics (2016)

  4. No Access

    Article

    The aromatic peroxygenase from Marasmius rutola—a new enzyme for biosensor applications

    The aromatic peroxygenase (APO; EC 1.11.2.1) from the agraric basidomycete Marasmius rotula (MroAPO) immobilized at the chitosan-capped gold-nanoparticle-modified glassy carbon electrode displayed a pair of redox...

    Aysu Yarman, Glenn Gröbe, Bettina Neumann… in Analytical and Bioanalytical Chemistry (2012)

  5. No Access

    Article

    Can peroxygenase and microperoxidase substitute cytochrome P450 in biosensors

    Aromatic peroxygenase (APO) from the basidiomycetous mushroom Agrocybe aegerita (AaeAPO) and microperoxidases (MPs) obtained from cytochrome c exhibit a broad substrate spectrum including hydroxylation of selecte...

    Aysu Yarman, Lei Peng, Yunhua Wu, Amay Bandodkar… in Bioanalytical Reviews (2011)

  6. Article

    Open Access

    High-yield production of aromatic peroxygenase by the agaric fungus Marasmius rotula

    An extracellular peroxygenase from Marasmius rotula was produced in liquid culture, chromatographically purified and partially characterized. This is the third aromatic peroxygenase (APO) that has been characteri...

    Glenn Gröbe, René Ullrich, Marek J Pecyna, Danuta Kapturska… in AMB Express (2011)

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  7. No Access

    Article

    Molecular characterization of aromatic peroxygenase from Agrocybe aegerita

    Recently, a novel group of fungal peroxidases, known as the aromatic peroxygenases (APO), has been discovered. Members of these extracellular biocatalysts produced by agaric basidiomycetes such as Agrocybe aegeri...

    Marek J. Pecyna, René Ullrich, Britta Bittner… in Applied Microbiology and Biotechnology (2009)

  8. No Access

    Article

    Hydroxylation of naphthalene by aromatic peroxygenase from Agrocybe aegerita proceeds via oxygen transfer from H2O2 and intermediary epoxidation

    Agrocybe aegerita peroxidase/peroxygenase (AaP) is an extracellular fungal biocatalyst that selectively hydroxylates the aromatic ring of naphthalene. Under alkaline conditions, the reaction proceeds via the form...

    Martin Kluge, René Ullrich, Christoph Dolge… in Applied Microbiology and Biotechnology (2009)

  9. Article

    The non-communicating rudimentary horn: diagnostic and therapeutic challenges

    We describe the case of a 23-year-old nulligravid woman who complained of increasing post-menstrual lower abdominal pain. She used contraceptives permanently for three months and was referred with a sub-mucosa...

    Katrin Scheibner, Annette Kuhn, Luigi Raio, Hermann Brühwiler… in Gynecological Surgery (2007)

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  10. No Access

    Article

    Spectrophotometric assay for detection of aromatic hydroxylation catalyzed by fungal haloperoxidase–peroxygenase

    Agrocybe aegerita peroxidase (AaP) is a versatile heme-thiolate protein that can act as a peroxygenase and catalyzes, among other reactions, the hydroxylation of aromatic rings. This paper reports a rapid and sel...

    Martin G. Kluge, René Ullrich, Katrin Scheibner… in Applied Microbiology and Biotechnology (2007)

  11. No Access

    Article

    Enzymatische Umsetzung von Arsenkampfstoffen durch das Pilzenzym Mangan-Peroxidase

    Das Pilzenzym Mangan-Peroxidase wurde eingesetzt, um verschiedene Arsenkampfstoffe in zellfreien Reaktionsansätzen (in vitro) abzubauen. Die Konzentrationsabnahme der Arsenkampfstoffe wurde nach Thiol-Derivatisie...

    Rainer Haas, Katrin Scheibner… in Umweltwissenschaften und Schadstoff-Forsch… (2003)

  12. No Access

    Article

    Transformation and mineralization of 2,4,6-trinitrotoluene (TNT) by manganese peroxidase from the white-rot basidiomycete Phlebia radiata

    The degradation of the nitroaromatic pollutant 2,4,6-trinitrotoluene (TNT) by the manganese-dependent peroxidase (MnP) of the white-rot fungus Phlebia radiata and the main reduction products formed were invest...

    Benoît Van Aken, Martin Hofrichter, Katrin Scheibner, Annele I. Hatakka… in Biodegradation (1999)