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Article
Open AccessPRMT2 promotes HIV-1 latency by preventing nucleolar exit and phase separation of Tat into the Super Elongation Complex
The HIV-1 Tat protein hijacks the Super Elongation Complex (SEC) to stimulate viral transcription and replication. However, the mechanisms underlying Tat activation and inactivation, which mediate HIV-1 produc...
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Article
Open AccessR-loop-dependent promoter-proximal termination ensures genome stability
The proper regulation of transcription is essential for maintaining genome integrity and executing other downstream cellular functions1,2. Here we identify a stable association between the genome-stability regula...
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Article
CDK13 phosphorylates the translation machinery and promotes tumorigenic protein synthesis
Cyclin-dependent kinase 13 (CDK13) has been suggested to phosphorylate RNA polymerase II and is involved in transcriptional activation. However, whether CDK13 catalyzes other protein substrates and how CDK13 c...
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Article
Open AccessG-quadruplexes sense natural porphyrin metabolites for regulation of gene transcription and chromatin landscapes
G-quadruplexes (G4s) are unique noncanonical nucleic acid secondary structures, which have been proposed to physically interact with transcription factors and chromatin remodelers to regulate cell type-specifi...
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Article
Open AccessPCGF6 controls neuroectoderm specification of human pluripotent stem cells by activating SOX2 expression
Polycomb group (PcG) proteins are known to repress developmental genes during embryonic development and tissue homeostasis. Here, we report that PCGF6 controls neuroectoderm specification of human pluripotent ...
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Protocol
Genome-Wide Native R-Loop Profiling by R-Loop Cleavage Under Targets and Tagmentation (R-Loop CUT&Tag)
R-loops are three-stranded nucleic acid structures that consist of a DNA–RNA hybrid and a displaced single-stranded DNA. R-loops occur during transcription and participate in multiple physiological processes s...
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Article
HERG1 K+ channels on the leukemic cells mediated angiogenesis in vitro
Human ether-a-go-go-related gene (HERG1) K+ channels are overexpressed in leukemia, which contributes to neoangiogenesis. The purpose of this study was to investigate the role of HERG1 K+ channels on leukemia ang...
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Article
Imatinib has the potential to exert its antileukemia effects by down-regulating hERG1 K+ channels in chronic myelogenous leukemia
Imatinib is a powerful protein tyrosine kinase (PTK) inhibitor that specifically targets BCR-ABL, KIT, and PDGFR kinases, has become the current first-line therapy for all newly diagnosed chronic myeloid leuke...
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Article
A Bipartite Recombinant Yeast System for the Identification of Subtype-Selective Estrogen Receptor Ligands
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Article
A Bipartite Recombinant Yeast System for the Identification of Subtype-Selective Estrogen Receptor Ligands
Since the estrogen receptor α (ERα) and β (ERβ) are thought to mediate different biological effects, there is intense interest in designing or screening subtype-selective ER ligands. Here, we constructed a bio...
