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  1. No Access

    Article

    DNA Fingerprinting with the oligonucleotide probe (CAC)5/(GTG)5: somatic stability and germline mutations

    DNA fingerprints were generated from various human somatic tissues and from peripheral blood of 179 children and their 80 parents using (CAC)5/(GTG)5 oligonucleotide probes. Whereas somatic stability of the finge...

    P. Nürnberg, L. Roewer, Heidemarie Neitzel, K. Sperling, Anita Pöpperl… in Human Genetics (1989)

  2. No Access

    Article

    Prematurely condensed human sperm chromosomes after in vitro fertilization (IVF)

    During an in vitro fertilization (IVF) program 122 inseminated eggs showing polar body extrusion, but neither formation of pronuclei nor cell cleavage were analysed cytogenetically. Nine of these eggs showed p...

    H. Schmiady, K. Sperling, H. Kentenich, M. Stauber in Human Genetics (1986)

  3. No Access

    Article

    Complementation studies between Fanconi's anemia cells with different DNA repair characteristics

    Hybrids were performed between cell lines derived from four patients with Fanconi's anemia in which different biochemical lesions have been postulated. Complementation studies in these hybrids based on the rat...

    Sabine Zakrzewski, M. Koch, K. Sperling in Human Genetics (1983)

  4. No Access

    Article

    Analysis of heterogeneity in Fanconi's anemia patients of different ethnic origin

    Complementation studies based on mitomycin C sensitivity were performed on somatic cell hybrids between cells of a German patient with Fanconi's anemia and a Turkish, Arab, and Black African proband, respectiv...

    Sabine Zakrzewski, K. Sperling in Human Genetics (1982)

  5. No Access

    Article

    Rabl's model of the interphase chromosome arrangement tested in Chinise hamster cells by premature chromosome condensation and laser-UV-microbeam experiments

    In 1885 Carl Rabl published his theory on the internal structure of the interphase nucleus. We have tested two predictions of this theory in fibroblasts grown in vitro from a female Chinese hamster, namely (1)...

    T. Cremer, C. Cremer, H. Baumann, E. K. Luedtke, K. Sperling, V. Teuber… in Human Genetics (1982)

  6. No Access

    Article

    Antagonistic effect of cocultivation on mitomycin C-induced aberration rate in cells of a patient with Fanconi's anemia and in Chinese hamster ovary cells

    The rate of spontaneous chromosomal aberrations in fibroblasts of a patient with Fanconi's anemia was slightly reduced after cocultivation with Chinese hamster ovary (CHO) cells. However, after mitomycin C tre...

    Sabine Zakrzewski, K. Sperling in Human Genetics (1980)

  7. No Access

    Article

    Genetic heterogeneity of Fanconi's anemia demonstrated by somatic cell hybrids

    Cells of patients with Fanconi's anemia (FA) are characterized by their high mitomycin C sensitivity. This specific response was used to study the question of heterogeneity in cell hybrids. After fusion of som...

    Sabine Zakrzewski, K. Sperling in Human Genetics (1980)

  8. No Access

    Article

    Activity of rRNA genes in cells of a patient with Down syndrome mosaic

    Disomic and trisomic cells of a patient with Down syndrome mosaic were used to study the effect of the additional chromosome 21 against an identical genetic background. The frequency of Ag staining and the par...

    R. -D. Wegner, P. Aldenhoff, K. Sperling in Human Genetics (1980)

  9. No Access

    Article

    Staining of the spindle apparatus in human lymphocyte and fibroblast cultures

    The spindle apparatus of human mitoses can be preferentially stained with Giemsa if the cells are fixed with methanol-acetic acid and treated for about 15 min with 0.1xSSC at 92°C and for 18 h with 2xSSC at 60°C.

    F. Pera, K. Sperling in Human Genetics (1976)

  10. No Access

    Article

    Cytogenetic investigations in a new case of Bloom's syndrome

    Cytogenetic studies of an 8-year-old caucasian girl with typical but mild manifestation of Bloom's syndrome showed a characteristic increase of homologous chromatid translocations and prematurely condensed chr...

    K. Sperling, U. Goll, J. Kunze, E. -K. Lüdtke, M. Tolksdorf, G. Obe in Human Genetics (1976)

  11. No Access

    Article

    Frequency and distribution of sister-chromatid exchanges in a case of Fanconi's anemia

    In lymphocytes of a 7-year-old boy with Fanconi's anemia the frequencies and sites of sister-chromatid exchanges (SCE) were studied with the BrdU-Giemsa method. The average frequency of SCE (8.8 per metaphase)...

    K. Sperling, R.-D. Wegner, H. Riehm, G. Obe in Humangenetik (1975)

  12. No Access

    Article

    Relative DNA content of human euchromatin and heterochromatin after G, C and giemsa 11 banding

    Human chromosomes and interphase nuclei labeled with 3H-thymidine and treated with the ASG and trypsin technique for G banding show no DNA loss. However, after G11 and C banding significantly more DNA is removed ...

    H. Schmiady, R. -D. Wegner, K. Sperling in Humangenetik (1975)

  13. No Access

    Article

    A rapid banding technique for routine use in human and comparative cytogenetics

    A simple combined enzyme-stain technique is described which gives within 20 min clear banding patterns in mammalian chromosomes.

    K. Sperling, R. Wiesner in Humangenetik (1972)

  14. No Access

    Article

    Pränatale Chromosomenanalyse mit Mosaikbefund 46,XX/92,XXXX

    The chromosome analysis from cells of the amniotic fluid performed in a 28 year old woman in the 30th week of pregnancy revealed a mosaic of female diploid and tetraploid metaphases. Lymphocyte cultures from t...

    K. Sperling, E. Saling in Humangenetik (1971)