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Article
DNA Fingerprinting with the oligonucleotide probe (CAC)5/(GTG)5: somatic stability and germline mutations
DNA fingerprints were generated from various human somatic tissues and from peripheral blood of 179 children and their 80 parents using (CAC)5/(GTG)5 oligonucleotide probes. Whereas somatic stability of the finge...
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Article
Prematurely condensed human sperm chromosomes after in vitro fertilization (IVF)
During an in vitro fertilization (IVF) program 122 inseminated eggs showing polar body extrusion, but neither formation of pronuclei nor cell cleavage were analysed cytogenetically. Nine of these eggs showed p...
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Article
Complementation studies between Fanconi's anemia cells with different DNA repair characteristics
Hybrids were performed between cell lines derived from four patients with Fanconi's anemia in which different biochemical lesions have been postulated. Complementation studies in these hybrids based on the rat...
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Article
Analysis of heterogeneity in Fanconi's anemia patients of different ethnic origin
Complementation studies based on mitomycin C sensitivity were performed on somatic cell hybrids between cells of a German patient with Fanconi's anemia and a Turkish, Arab, and Black African proband, respectiv...
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Article
Rabl's model of the interphase chromosome arrangement tested in Chinise hamster cells by premature chromosome condensation and laser-UV-microbeam experiments
In 1885 Carl Rabl published his theory on the internal structure of the interphase nucleus. We have tested two predictions of this theory in fibroblasts grown in vitro from a female Chinese hamster, namely (1)...
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Article
Antagonistic effect of cocultivation on mitomycin C-induced aberration rate in cells of a patient with Fanconi's anemia and in Chinese hamster ovary cells
The rate of spontaneous chromosomal aberrations in fibroblasts of a patient with Fanconi's anemia was slightly reduced after cocultivation with Chinese hamster ovary (CHO) cells. However, after mitomycin C tre...
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Article
Genetic heterogeneity of Fanconi's anemia demonstrated by somatic cell hybrids
Cells of patients with Fanconi's anemia (FA) are characterized by their high mitomycin C sensitivity. This specific response was used to study the question of heterogeneity in cell hybrids. After fusion of som...
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Article
Activity of rRNA genes in cells of a patient with Down syndrome mosaic
Disomic and trisomic cells of a patient with Down syndrome mosaic were used to study the effect of the additional chromosome 21 against an identical genetic background. The frequency of Ag staining and the par...
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Article
Staining of the spindle apparatus in human lymphocyte and fibroblast cultures
The spindle apparatus of human mitoses can be preferentially stained with Giemsa if the cells are fixed with methanol-acetic acid and treated for about 15 min with 0.1xSSC at 92°C and for 18 h with 2xSSC at 60°C.
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Article
Cytogenetic investigations in a new case of Bloom's syndrome
Cytogenetic studies of an 8-year-old caucasian girl with typical but mild manifestation of Bloom's syndrome showed a characteristic increase of homologous chromatid translocations and prematurely condensed chr...
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Article
Frequency and distribution of sister-chromatid exchanges in a case of Fanconi's anemia
In lymphocytes of a 7-year-old boy with Fanconi's anemia the frequencies and sites of sister-chromatid exchanges (SCE) were studied with the BrdU-Giemsa method. The average frequency of SCE (8.8 per metaphase)...
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Article
Relative DNA content of human euchromatin and heterochromatin after G, C and giemsa 11 banding
Human chromosomes and interphase nuclei labeled with 3H-thymidine and treated with the ASG and trypsin technique for G banding show no DNA loss. However, after G11 and C banding significantly more DNA is removed ...
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Article
A rapid banding technique for routine use in human and comparative cytogenetics
A simple combined enzyme-stain technique is described which gives within 20 min clear banding patterns in mammalian chromosomes.
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Article
Pränatale Chromosomenanalyse mit Mosaikbefund 46,XX/92,XXXX
The chromosome analysis from cells of the amniotic fluid performed in a 28 year old woman in the 30th week of pregnancy revealed a mosaic of female diploid and tetraploid metaphases. Lymphocyte cultures from t...