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Article
Open AccessTime course of the sensitivity and specificity of anti-SARS-CoV-2 IgM and IgG antibodies for symptomatic COVID-19 in Japan
The accurate and prompt diagnosis of SARS-CoV-2 infection is required for the control and treatment of the coronavirus infection disease 2019 (COVID-19). In this study, we aimed to investigate the time courses...
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Article
Open AccessIdentification of a novel strong promoter from the anhydrobiotic midge, Polypedilum vanderplanki, with conserved function in various insect cell lines
Larvae of the African midge Polypedilum vanderplanki (Diptera: Chironomidae) show a form of extreme desiccation tolerance known as anhydrobiosis. The cell line Pv11 was recently established from the species, and ...
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Article
Establishment of gene transfer and gene silencing methods in a desiccation-tolerant cell line, Pv11
Larvae of the African midge Polypedilum vanderplanki show extreme desiccation tolerance, known as anhydrobiosis. Recently, the cultured cell line Pv11 was derived from this species; Pv11 cells can be preserved in...
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Article
Diversity of the expression profiles of late embryogenesis abundant (LEA) protein encoding genes in the anhydrobiotic midge Polypedilum vanderplanki
In the anhydrobiotic midge Polypedilum vanderplanki , LEA family proteins are likely to play distinct temporal and spatial roles in the larvae throughout the...
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Article
Open AccessEnzymatic activity of a subtilisin homolog, Tk-SP, from Thermococcus kodakarensisin detergents and its ability to degrade the abnormal prion protein
Tk-SP is a member of subtilisin-like serine proteases from a hyperthermophilic archaeon Thermococcus kodakarensis. It has been known that the hyper-stable protease, Tk-SP, could exhibit enzymatic activity even at...
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Article
Open AccessEvolution and thermodynamics of the slow unfolding of hyperstable monomeric proteins
The unfolding speed of some hyperthermophilic proteins is dramatically lower than that of their mesostable homologs. Ribonuclease HII from the hyperthermophilic archaeon Thermococcus kodakaraensis (Tk-RNase HII) ...
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Article
Efficacy and safety of mizoribine for the treatment of Sjögren's syndrome: a multicenter open-label clinical trial
This multicenter clinical trial was performed to evaluate the efficacy and safety of mizoribine for the treatment of Sjögren's syndrome. Fifty-nine patients with a definite diagnosis of Sjögren's syndrome rece...
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Article
Coexistence of nodular regenerative hyperplasia of the liver and pulmonary arterial hypertension in patients with connective tissue diseases: report of three cases and review of the literature
Nodular regenerative hyperplasia of the liver (NRH) is known to be a rare condition in patients with connective tissue diseases (CTD). In this report, we document three patients with CTD who had both NRH and p...
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Article
Open AccessNOS2polymorphisms associated with the susceptibility to pulmonary arterial hypertension with systemic sclerosis: contribution to the transcriptional activity
Systemic sclerosis (SSc) is a connective tissue disease characterized by tissue fibrosis. One of several complications of SSc, pulmonary arterial hypertension (PAH) can be refractory to treatment, both novel a...
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Article
Ground uplift detected by precise leveling in the Ontake earthquake swarm area, central Japan in 2002–2004
Repeated precise leveling in the earthquake swarm area of Ontake, central Japan has revealed uplift of 3–6 mm in proximity to the epicentral region of the most active earthquake cluster in 2002–2004. Although ...
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Article
Prospective study of high-dose intravenous immunoglobulin for the treatment of steroid-resistant polymyositis and dermatomyositis
High-dose intravenous immunoglobulin (IVIG) therapy has been effective in treating many autoimmune and systemic inflammatory diseases. In the present prospective study, we evaluated the efficacy of IVIG for pa...
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Article
Epstein-Barr Virus-Associated B-Cell Type Non-Hodgkin’s Lymphoma with Concurrent p53 Protein Expression in a Rheumatoid Arthritis Patient Treated with Methotrexate
A Japanese male patient received various medications for his long-standing rheumatoid arthritis (stage IV, class II). He developed a mass on the right anterior chest wall after being treated with methotrexate ...
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Article
T-Cell Type Acute Lymphoblastic Leukemia Following Cyclosporin A Therapy for Aplastic Anemia
Cyclosporin A (CsA) is used to prevent rejection in transplantation and to treat autoimmune and hematologic diseases such as aplastic anemia. However, the tumor growth-promoting effect of CsA remains controver...
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Article
Variation of frontal P20 potential due to rotation of the N20-P20 dipole moment of SEPs
We have applied the artificial neural network method to estimate the N20-P20 dipole from scalp SEP potentials, and have investigated the rotation of the dipole moment caused by the compression effect of a brai...
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Article
Artificial neural networks for source localization in the human brain
Source localization in the brain remains an ill-posed problem unless further constraints about the type of sources and the head model are imposed. Human head is modeled in various ways depending critically on ...
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Article
Alloantigens expressed on a subset of unstimulated human T lymphocytes that generate suppressor function
Human alloantisera were tested for antibodies reacting with T-cell subpopulations. T-cell subsets were separated using the monoclonal antibodies OKT4 and OKT8. Five sera reacting with the T4-T8+ subset and two...
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Changes of Antimicrobial Susceptibility of Anaerobic Bacteria from Clinical Specimen
There has been a rather remarkable upsurge of interest in anaerobes and in the anaerobic infections in recent years. Especially Bacteroides species occupies a preeminent position in anaerobic infections, becau...
