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  1. No Access

    Protocol

    Nonlocalized Searching of HCD Data for Fast and Sensitive Identification of ADP-Ribosylated Peptides

    ADP-ribosylation is a technically challenging PTM which has just emerged into the field of PTM-specific proteomics. But this fragile modifier requires special treatment on both a data acquisition and data proc...

    Thomas Colby, Juan José Bonfiglio… in ADP-ribosylation and NAD+ Utilizing Enzymes (2018)

  2. Article

    Open Access

    cAMP-dependent cell differentiation triggered by activated CRHR1 in hippocampal neuronal cells

    Corticotropin-releasing hormone receptor 1 (CRHR1) activates the atypical soluble adenylyl cyclase (sAC) in addition to transmembrane adenylyl cyclases (tmACs). Both cAMP sources were shown to be required for ...

    Carolina Inda, Juan José Bonfiglio, Paula A. dos Santos Claro… in Scientific Reports (2017)

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    Article

    Serine is a new target residue for endogenous ADP-ribosylation on histones

    ADP-ribosylation is a post-translational protein modification that regulates numerous cellular pathways. An approach involving histone purification, partial filter-aided digestion and ETD mass spectrometry re...

    Orsolya Leidecker, Juan José Bonfiglio, Thomas Colby, Qi Zhang… in Nature Chemical Biology (2016)